Phage Hunters Spring 2017
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| unacceptable | BPT7:V5557 | 2017-04-16 14:38:34 CDT | GO:0006944 cellular membrane fusion (P) | PMID:22566619 | ECO:0000316 genetic interaction evidence used in manual assertion | T7 replication yielding HN-S inhibition and production. Inhibition of phage growth in cell.
Assists in fusion and plating
| challenge |
| unacceptable | BPT7:V5557 | 2017-04-16 14:38:34 CDT | GO:0006944 cellular membrane fusion (P) | PMC45959:https://www-ncbi-nlm-nih-gov.proxy.library.vcu.edu/pmc/articles/PMC45959/pdf/pnas01464-0136.pdf | ECO:0000316 genetic interaction evidence used in manual assertion | DNA compaction and supercoiling. Specific binding to H-NS inhibits and effects dna plaque, also binds to A rexAB
system
| challenge |
| unacceptable | BPMD2:LYSB | 2017-05-08 21:50:49 CDT | GO:0044659 cytolysis by virus of host cell (P) | other:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774421/figure/F3/ | ECO:0000255 match to sequence model evidence used in manual assertion | Of the 253 residue for D29 LysB, 170 are typical for the homologic match to the alpha/beta hydrolase. this structure is composed of a beta sheet in between 2 alpha sheets.
The remaining 81- residues form a C-terminal alpha helix that has a backside attachment to the Beta sheet. the structure and polarization of the end of the C-terminal yields a structure where Gly117-ASN118-PRO119 allowing a 180 degree turn underneath ASP 166 with 117 and 119 forming hydrogen bonds with met120, Arg121, and Asp160 allowing rotation that helps facilitate the catalytic state of the release of mycolic acid from the host cell membrane.
The composition of the residues leads a 21% identity with amino acids. However, it yields a high Z-score of 20.3 for comparison in Dali. This lack of identity is possibly due to the 55 residues that are high in glycine residues that lead to the structure in between the second and third helices.
| challenge |
| requireschanges | BPMD2:LYSB | 2017-05-08 22:36:52 CDT | GO:0044659 cytolysis by virus of host cell (P) | PMID:19555454 | ECO:0000316 genetic interaction evidence used in manual assertion | Figure 4-
After determining if M. smegmatis is an appropriate target host cell and it's mycolyl-arabinogalactan-peptidoglycan membrane is hydrolyzed, testing the proposed function of a Serine esterase was tested by Chromatography to determine the release of Mycolic acids. Each time period is noted in figure A. Figure A and B are time and concentration dependent of the release of lipids. This is designed to mock the mycolic acid release known from treating the membrane with tetrabutylammonium. Figure C is classifying the concentration of the specific lipids released in figure A and B. Figure D is the most critical, by measuring the mutant that had the serine 82 residue removed, an important catalyst, along with test of other chemicals and controls, it is evident that Lyse B intact with the Serine and other catalysts intact are essential to releasing the free mycolic acids in the cell membrane.
| challenge |
acceptable:0
unacceptable:3
requires_changes:1
flagged:0
Annotations challenged by Crowap
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
|---|
0 annotations fixed by Crowap
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