CACAO Spring 2017
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| acceptable | MYCTU:PKNA | 2017-04-06 20:00:43 CDT | GO:0008360 regulation of cell shape (P) | PMID:25713147 | ECO:0000315 mutant phenotype evidence used in manual assertion | Mycobacterium tuberculosis (strain H37Rv)
Serine/threonine-protein kinase A
Figure 3B: The cell morphologies of Mycobacterium tuberculosis strains H37Rv (wild-type), Rv-pptr-AB (pknA and pknB conditional mutant, pknA-pknB expression was under the regulation of a pristinamycin inducible promoter [pptr]), and Rv-pptr-AB::PknB (electroporated with pCiT-PknB, containing anhydrotetracycline [ATc] inducible PknB) were examined using scanning electron microscopy. H37Rv cultures were grown for 4 days in the absence of any inducer. Rv-pptr-AB cultures were grown either in the presence or absence of pristinamycin for 2 and 4 days. Rv-pptr-AB::PknB cultures were grown in the presence of ATc (in the absence of pristinamycin) for 2 and 4 days. After 4 days of growth, Rv-pptr-AB and Rv-pptr-AB::PknB cells showed signs of substantial cell-cell fusion and cell lysis, relative to the wild-type H37Rv. These results suggest that PknA plays a role in regulating cell morphology in Mycobacterium tuberculosis.
| challenge |
acceptable:1
unacceptable:0
requires_changes:0
flagged:0
Annotations challenged by Austinhewitt
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
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0 annotations fixed by Austinhewitt
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