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Cacao
| GO:0017111 | nucleoside-triphosphatase activity | PMID:26277776 | ECO:0001258 IDA: Inferred from Direct Assay | Molecular Function | The bacteriophage BFK20 of the Brevibacterium flavum has the gp43-1 protein, which contains a prim/pol domain at the N-terminus and a helicase domain at the C-terminus. Gp43-1 was isolated, and an NTPase activity assay was utilized to determine its function as an NTPase. The reaction mixture contained ssDNA or dsDNA, and NTP or dNTP, and the reaction started when the NTPase was added to the mixture. A control was also prepared, in which the NTPase was substituted with storage buffer. The amount of phosphate released by the gp43-1 protein was quantified through a colorimetric spectrophotometer analysis; the results of this data is depicted in Figure 6. The amount of phosphate in the reaction mixture was measured at the beginning of the experiment and at set intervals. The two quantities were subtracted to determine the amount of phosphate produced by the NTPase at a certain time. After gathering and analyzing the data as shown in Figure 6, it was concluded that gp43-1 was able to hydrolyze NTPs. | complete | ||
| This annotation made on page: 9CAUD:Q3V5F2 By: ASimpson (group Team Purple B 2019) on 2019-03-25 10:42:52 CDT. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Public Assessment | Ivanerill | 2019-03-27 10:05:37 CDT | No notes given. | Acceptable ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original | ||||||||||||||||||||||
| Public Assessment | DanielRenfro | 2019-03-27 09:49:40 CDT | This annotation has been flagged because it has been edited since last assessment
| Flagged | ||||||||||||||||||||||
| Public Assessment | DanielRenfro | 2019-03-27 09:15:02 CDT | This annotation has been flagged because it has been edited since last assessment
| Flagged | ||||||||||||||||||||||
| Public Assessment | Ivanerill | 2019-03-25 12:40:39 CDT | No notes given. | Acceptable ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original |
