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Cacao

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GO:0034291canonical holin activityPMID:12533453IDA: Inferred from Direct Assay F
This annotation made on page: LAMBD:HOLIN
By: CMattson (group Team Blue B 2018) on 2018-03-14 09:03:56 CDT.



TeamPoints
Team Blue B 20185




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment		Ivanerill2018-04-15 14:59:49 CDTNo notes given.Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2018-03-30 07:27:52 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0045918 negative regulation of cytolysis PMID:21187415 IDA: Inferred from Direct Assay P Figure 3. S105, holin, and S107, antiholin, are two transmembrane proteins produced by alternate starts on gene S in phage lambda. S105 accumulates into rafts, which are associated with the formation of large holes in the membrane. These holes allow endolysin to reach the peptidoglycan and result in cytolysis. S105 has three transmembrane domains. The difference between S105 and S107 is an extra N-terminal positive charge on S107, which prevents the first transmembrane domain from entering the membrane. The researchers created a mutant allele of S105 with the first transmembrane domain deleted, such that it would act like S107, and fused it to mCherryFP. Through deconvolution fluorescence imaging, they showed that the fusion protein blocked S105 raft formation, suggesting that antiholins prevent lysis by blocking the formation of holin rafts. complete
CACAO 13115
on LAMBD:HOLIN
Flagged
Public
Assessment		Ivanerill2018-03-28 10:58:32 CDT

This is not IMP. Note that there are other possible annotations in the paper too (e.g. celllular component)

Requires Changes
✔ Protein
✔ Publication
✔ Go term
✗ Evidence
Public
Assessment		DanielRenfro2018-03-24 11:35:48 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0045918 negative regulation of cytolysis PMID:21187415 IMP: Inferred from Mutant Phenotype P Figure 3. S105, holin, and S107, antiholin, are two transmembrane proteins produced by alternate starts on gene S in phage lambda. S105 accumulates into rafts, which are associated with the formation of large holes in the membrane. These holes allow endolysin to reach the peptidoglycan and result in cytolysis. S105 has three transmembrane domains. The difference between S105 and S107 is an extra N-terminal positive charge on S107, which prevents the first transmembrane domain from entering the membrane. The researchers created a mutant allele of S105 with the first transmembrane domain deleted, such that it would act like S107, and fused it to mCherryFP. Through deconvolution fluorescence imaging, they showed that the fusion protein blocked S105 raft formation, suggesting that antiholins prevent lysis by blocking the formation of holin rafts. complete
CACAO 13115
on LAMBD:HOLIN
Flagged
Public
Assessment		Ivanerill2018-03-23 05:41:12 CDT

Make sure the annotation pertains to the holin, not the lysin, as the paper seems to imply. Also, the figure cited uses a mutant to prove their point. Is holin activity demonstrated by the experiment? If so, you may need to make a stronger case with your notes.

Requires Changes
✗ Protein
✔ Publication
✔ Qualifier
✗ Go term
✗ Evidence
✔ With/From
✗ Notes
✔ Unique/Original


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