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GO:0019076viral release from host cellPMID:12533453IDA: Inferred from Direct Assay P
This annotation made on page: LAMBD:ENLYS
By: CMattson (group Team Blue B 2018) on 2018-03-04 23:17:00 CST.



TeamPoints
Team Blue B 20185




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment
Scaruso2018-03-12 08:35:56 CDTNo notes given.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2018-03-11 20:39:39 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0044659 cytolysis by virus of host cell PMID:28637001 IDA: Inferred from Direct Assay P The R lysis cassette, including R endolysin and S holin genes was synthesized and inserted into a plasmid under regulation of arabinose and temperature sensitive promoters. The plasmid was introduced into Δasd E. coli χ6212 and the construct was named JOL1675. Under conditions inducing expression of the plasmid, presence of the R endolysin was confirmed by Western Blot and shown in Figure 2. A band at 17.7 kDa, the estimated size of the endolysin, was seen in cells with the plasmid expressed, but not in cells grown under conditions repressing expression. Lysis efficiency was confirmed by monitoring the number of viable cells, which declined to 0 after 48 hours of lysis, as shown in Figure 4. No viable cells remained, indicating that the endolysin effectively ruptured and killed the hosts. complete
CACAO 13107
on LAMBD:ENLYS
Flagged
Public
Assessment
DanielRenfro2018-03-10 10:54:06 CST

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0019076 viral release from host cell PMID:12533453 IDA: Inferred from Direct Assay P Even when the lacZ gene was added to the endolysin R gene, making the protein ~500 kDa, the lesions created by holin allowed the protein to pass through the membrane, effectively lysing host cells. complete
CACAO 13107
on LAMBD:ENLYS
Flagged
Public
Assessment
Ivanerill2018-03-08 15:26:23 CST

You want a point to the specific evidence in the manuscript (table, figure) that shows the mapping between your protein and the GO term, and explain the rationale.

Currently you have: "Even when the lacZ gene was added to the endolysin R gene, making the protein ~500 kDa, the lesions created by holing allowed the protein to pass through the membrane, effectively lysing host cells." But the lacZ fusion is not a factor in demonstrating the activity. And where are you referring to in the paper? You should also look at other possible GO terms, since maybe what is demonstrated is not release, but just lysis? e.g. GO:0044659

Requires Changes
Protein
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Scaruso2018-03-08 14:13:00 CST

This is a fine paper, but you should include which figure shows that the R protein (endolysin) is shown cause GO:0019076, viral release from host cell, by IDA. Presumably, you intend Fig. 1, as it is probably the best that fits your description. You may also consider using a later one for an IMP in another annotation. Also, consider looking at a GO term one step more detailed: GO:0044659, cytolysis by virus of host cell

Requires Changes
Protein
Publication
Qualifier
Evidence
With/From
Notes
Unique/Original