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Cacao

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GO:1990443peptidyl-threonine autophosphorylationPMID:25713147IMP: Inferred from Mutant Phenotype P
This annotation made on page: MYCTU:PKNA
By: Joshuakrank (group Team Moldevort) on 2017-03-23 09:57:15 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeSarahCouv,
Team Lily Whale
2017-03-28 20:39:55 CDT

The notes are missing the organism for the paper. Corrected notes should be:

Organism: Mycobacterium tuberculosis H37Rv Protein: Serine/threonine-protein kinase PknA Fig 9(b + d): The protein phosphokinase A (pknA) was detected in a wild strain of Mycobacterium tuberculosis H37Rv using the antibody alpha-Thr(P), which picks up the threonine residues 172 and 174 in the pkna activation loop. PknA was not detected in the strain pknA(K42M), a strain where kinases were inactivated. This suggested that kinases (like pknA) were being activated by auto-phosphorylation. Fig 9e: In a strain in which the transcription level of pknB is under the control of a pristinamycin inducible promoter, the level of pknA is not impacted whether the strain is grown in the presence or absence of the inducer. This is indicative that pknA is most likely activated through auto-phosphorylation and is independent of pknB

0
Private
Assessment
Suzialeksander2017-04-07 16:33:20 CDTYou need to be an instructor to view these notes.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2017-03-28 20:39:55 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Public
Assessment
DanielRenfro2017-03-23 15:21:07 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:1990443 peptidyl-threonine autophosphorylation PMID:25713147 IMP: Inferred from Mutant Phenotype P Fig 9(b + d): The protein phosphokinase A (pknA) was detected in a wild strain Mycobacterium tuberculosis H37Rv using the antibody alpha-Thr(P), which picks up the threonine residues 172 and 174 in the pkna activation loop. PknA was not detected in the strain pknA(K42M), a strain where kinases were inactivated. This suggested that kinases (like pknA) were being activated by auto-phosphorylation.

Fig 9e: In a strain in which the transcription level of pknB is under the control of a pristinamycin inducible promoter, the level of pknA is not impacted whether the strain is grown in the presence or absence of the inducer. This is indicative that pknA is most likely activated through auto-phosphorylation and is independent of pknB||complete
CACAO 12340

on MYCTU:PKNA
Flagged
Private
Assessment
Suzialeksander2017-03-23 14:07:11 CDTYou need to be an instructor to view these notes.Requires Changes
Notes
Private
Assessment
Suzialeksander2017-03-23 13:59:35 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original