GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
TableEdit
PMID:20001858
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
See Help for Help on this wiki. See the documentation for how to use the table editor
| Citation |
Nygaard, TK, Pallister, KB, Ruzevich, P, Griffith, S, Vuong, C and Voyich, JM (2010) SaeR binds a consensus sequence within virulence gene promoters to advance USA300 pathogenesis. J. Infect. Dis. 201:241-54 |
|---|---|
| Abstract |
This investigation examines the role of the SaeR/S 2-component system in USA300, a prominent circulating clone of community-associated methicillin-resistant Staphylococcus aureus. Using a saeR/S isogenic deletion mutant of USA300 (USA300DeltasaeR/S) in murine models of sepsis and soft-tissue infection revealed that this sensory system is critical to pathogenesis of USA300 during both superficial and invasive infection. Oligonucleotide microarray and real-time reverse-transcriptase polymerase chain reaction identified numerous extracellular virulence genes that are down-regulated in USA300DeltasaeR/S. Unexpectedly, an up-regulation of mecA and mecR1 corresponded to increased methicillin resistance in USA300DeltasaeR/S. 5'-RACE analysis defined transcript start sites for sbi, efb, mecA, lukS-PV, hlb, SAUSA300_1975, and hla, to underscore a conserved consensus sequence within promoter regions of genes under strong SaeR/S transcriptional regulation. Electrophoretic mobility shift assay experiments illustrated direct binding of SaeR(His) to promoter regions containing the conserved consensus sequence. Collectively, the findings of this investigation demonstrate that SaeR/S directly interacts with virulence gene promoters to significantly influence USA300 pathogenesis. |
| Links |
PubMed PMC2798008 Online version:10.1086/649570 |
| Keywords |
Animals; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Community-Acquired Infections/microbiology; Disease Models, Animal; Electrophoretic Mobility Shift Assay; Gene Expression Profiling; Humans; Methicillin-Resistant Staphylococcus aureus/genetics; Methicillin-Resistant Staphylococcus aureus/pathogenicity; Mice; Oligonucleotide Array Sequence Analysis; Promoter Regions, Genetic/genetics; Sequence Deletion; Soft Tissue Infections/microbiology; Up-Regulation; Virulence Factors/metabolism |
| public |
| Cancel |
