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ECOLI:RNE

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Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

public

GO:0004519

endonuclease activity

PMID:9790196

ECO:0000314

F

Two forms of RNA were used, linear and circular. The circular RNA did not have a 5' end and thus could not be digested by RNase E as shown in Figure 2a. The circular RNA was then digested by RNase H and thus linearized as shown in Figure 2b. The now linearized RNA was then digested by RNase E which since the RNA was linear could be digested by RNase E as shown in Figure 2d and Figure 2e. The bands shown in Figures 2d,e are consistent with the digestion sites of both RNase H and RNase E.

complete

protected

enables

GO:0017151

DEAD/H-box RNA helicase binding

PMID:15236960

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P0A8J8

F

Seeded From UniProt

complete

protected

involved_in

GO:1902280

regulation of ATP-dependent RNA helicase activity

PMID:15236960

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

protected

enables

GO:0003723

RNA binding

PMID:15236960

ECO:0000315

mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

protected

enables

GO:0008312

7S RNA binding

PMID:15236960

ECO:0000315

mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

protected

involved_in

GO:0006364

rRNA processing

PMID:21873635

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG11299
PANTHER:PTN001246338

P

Seeded From UniProt

complete

protected

enables

GO:0042802

identical protein binding

PMID:18682225

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P21513

F

Seeded From UniProt

complete

protected

enables

GO:0042802

identical protein binding

PMID:15236960

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P21513

F

Seeded From UniProt

complete

protected

involved_in

GO:0051289

protein homotetramerization

PMID:14636052

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P21513

P

Seeded From UniProt

complete

protected

enables

GO:0008995

ribonuclease E activity

PMID:12417756

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

protected

involved_in

GO:0008033

tRNA processing

PMID:11871663

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

protected

involved_in

GO:0006402

mRNA catabolic process

PMID:1846032

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

protected

involved_in

GO:0006401

RNA catabolic process

PMID:8415644

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

protected

involved_in

GO:0000967

rRNA 5'-end processing

PMID:10329633

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

protected

enables

GO:0000287

magnesium ion binding

PMID:25404697

ECO:0000315

mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

protected

involved_in

GO:0090305

nucleic acid phosphodiester bond hydrolysis

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0004519

P

Seeded From UniProt

complete

protected

involved_in

GO:0090305

nucleic acid phosphodiester bond hydrolysis

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0004518

P

Seeded From UniProt

complete

protected

involved_in

GO:0090501

RNA phosphodiester bond hydrolysis

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0004540

P

Seeded From UniProt

complete

protected

involved_in

GO:0090501

RNA phosphodiester bond hydrolysis

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0008995

P

Seeded From UniProt

complete

protected

involved_in

GO:0090501

RNA phosphodiester bond hydrolysis

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0008995

P

Seeded From UniProt

complete

protected

involved_in

GO:0090502

RNA phosphodiester bond hydrolysis, endonucleolytic

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0004521

P

Seeded From UniProt

complete

protected

enables

GO:0003676

nucleic acid binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR003029

F

Seeded From UniProt

complete

protected

enables

GO:0003723

RNA binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR004659
InterPro:IPR019307

F

Seeded From UniProt

complete

protected

enables

GO:0004540

ribonuclease activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR004659

F

Seeded From UniProt

complete

protected

involved_in

GO:0006396

RNA processing

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR004659
InterPro:IPR028878

P

Seeded From UniProt

complete

protected

enables

GO:0008995

ribonuclease E activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR028878

F

Seeded From UniProt

complete

protected

part_of

GO:0005737

cytoplasm

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112759

C

Seeded From UniProt

complete

protected

enables

GO:0004521

endoribonuclease activity

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112759

F

Seeded From UniProt

complete

protected

involved_in

GO:0006402

mRNA catabolic process

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112759

P

Seeded From UniProt

complete

protected

part_of

GO:0009898

cytoplasmic side of plasma membrane

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112759

C

Seeded From UniProt

complete

protected

enables

GO:0000287

magnesium ion binding

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112759

F

Seeded From UniProt

complete

protected

involved_in

GO:0006364

rRNA processing

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112759

P

Seeded From UniProt

complete

protected

enables

GO:0003723

RNA binding

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112759

F

Seeded From UniProt

complete

protected

involved_in

GO:0008033

tRNA processing

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112759

P

Seeded From UniProt

complete

protected

enables

GO:0008270

zinc ion binding

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112759

F

Seeded From UniProt

complete

protected

part_of

GO:0005886

plasma membrane

GO_REF:0000037
GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1003
UniProtKB-KW:KW-0997
UniProtKB-SubCell:SL-0037

C

Seeded From UniProt

complete

protected

enables

GO:0003723

RNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0694

F

Seeded From UniProt

complete

protected

part_of

GO:0016020

membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0472

C

Seeded From UniProt

complete

protected

enables

GO:0046872

metal ion binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0479

F

Seeded From UniProt

complete

protected

enables

GO:0004519

endonuclease activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0255

F

Seeded From UniProt

complete

protected

involved_in

GO:0006364

rRNA processing

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0698

P

Seeded From UniProt

complete

protected

part_of

GO:0005737

cytoplasm

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0963
UniProtKB-SubCell:SL-0086

C

Seeded From UniProt

complete

protected

enables

GO:0016787

hydrolase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0378

F

Seeded From UniProt

complete

protected

enables

GO:0004518

nuclease activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0540

F

Seeded From UniProt

complete

protected

involved_in

GO:0008033

tRNA processing

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0819

P

Seeded From UniProt

complete




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