GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

TableEdit

Jump to: navigation, search

PMID:18950725

You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor

Citation

Koskinen, J, Karlsson, J and Olsson, PE (2009) Sox9a regulation of ff1a in zebrafish (Danio rerio) suggests an involvement of ff1a in cartilage development. Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 153:39-43

Abstract

The NR5A family of orphan nuclear receptors has been implicated in development of the vertebrate embryo, but their exact role remains largely unknown. To evaluate the regulation and developmental role for ff1a (NR5A2) in zebrafish (Danio rerio), we performed morpholino knockdown to block translation of the ff1a gene and the upstream located sox9a gene during embryogenesis. Using a newly developed antibody against Ff1a we could show that the ff1a morpholinos were functional and that a reduction in the expression of Ff1a correlated to altered phenotypes. The role of Sox9a in ff1a gene regulation and function was studied using sox9a morpholinos. Knock-down of sox9a resulted in abolished ff1a signals in the somites, mandibular arches and pharyngeal arches, while the pectoral fin signal remained. The reduction in Ff1a levels correlated to truncated tails and cranio-facial malformation. As Sox9a is involved in chondrocyte development we analysed for cartilage formation and found that blocking translation of either sox9a or ff1a also blocked cartilage formation. In light of the results, the present study suggests a novel function of ff1a in chondrocyte development.

Links

PubMed Online version:10.1016/j.cbpa.2008.10.003

Keywords

Alcian Blue; Animals; Blotting, Western; Cartilage/embryology; Cartilage/metabolism; Embryo, Nonmammalian/metabolism; Gene Expression Regulation, Developmental; Gonads/metabolism; In Situ Hybridization; Liver/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Receptors, Cytoplasmic and Nuclear/genetics; Receptors, Cytoplasmic and Nuclear/metabolism; SOX9 Transcription Factor/genetics; SOX9 Transcription Factor/metabolism; Zebrafish/embryology; Zebrafish/genetics; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism

public



Cancel