GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

TableEdit

Jump to: navigation, search

PMID:20010805

You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor

Citation

Mauvezin, C, Orpinell, M, Francis, VA, Mansilla, F, Duran, J, Ribas, V, Palacín, M, Boya, P, Teleman, AA and Zorzano, A (2010) The nuclear cofactor DOR regulates autophagy in mammalian and Drosophila cells. EMBO Rep. 11:37-44

Abstract

The regulation of autophagy in metazoans is only partly understood, and there is a need to identify the proteins that control this process. The diabetes- and obesity-regulated gene (DOR), a recently reported nuclear cofactor of thyroid hormone receptors, is expressed abundantly in metabolically active tissues such as muscle. Here, we show that DOR shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions, and re-localizes to autophagosomes on autophagy activation. We demonstrate that DOR interacts physically with autophagic proteins Golgi-associated ATPase enhancer of 16 kDa (GATE16) and microtubule-associated protein 1A/1B-light chain 3. Gain-of-function and loss-of-function studies indicate that DOR stimulates autophagosome formation and accelerates the degradation of stable proteins. CG11347, the DOR Drosophila homologue, has been predicted to interact with the Drosophila Atg8 homologues, which suggests functional conservation in autophagy. Flies lacking CG11347 show reduced autophagy in the fat body during pupal development. All together, our data indicate that DOR regulates autophagosome formation and protein degradation in mammalian and Drosophila cells.

Links

PubMed PMC2816618 Online version:10.1038/embor.2009.242

Keywords

Animals; Autophagy/genetics; Autophagy/physiology; Cell Line; Cell Nucleus/metabolism; Cytoplasm/metabolism; Diabetes Mellitus; Drosophila/anatomy & histology; Drosophila/genetics; Drosophila/growth & development; Drosophila/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Fat Body/metabolism; Fluorescent Antibody Technique; HeLa Cells; Humans; Microfilament Proteins/metabolism; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Microtubule-Associated Proteins/metabolism; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Obesity; Phagosomes/metabolism; Protein Binding; Protein Transport; Receptors, Thyroid Hormone/metabolism; Stress, Physiological

public



Cancel