GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search


You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor


Saitoh, T and Katoh, M (2001) Molecular cloning and characterization of human WNT8A. Int. J. Oncol. 19:123-7


WNT - beta-catenin - TCF pathway is involved in carcinogenesis and fetal development. Xenopus wnt-8 is one of the most potent Wnts with the capacity to activate beta-catenin - TCF pathway in the Xenopus axis duplication assay. Here, we have cloned and characterized WNT8A, a novel human homologue of Xenopus wnt-8. The WNT8A gene, consisting of at least 6 exons, was found to encode a 351-amino-acid polypeptide with the N-terminal signal peptide, three N-linked glycosylation sites, and conserved amino-acid residues of the WNT family. WNT8A showed 63.2% total-amino-acid identity to WNT8B. C-terminal region of WNT8A, WNT8B, WNT2, WNT2B1 and WNT2B2 were longer than that of other WNTs. Among various normal human tissues and 34 human cancer cells lines, the 3.5-kb WNT8A mRNA was detected only in a human teratocarcinoma cell line NT2. These results strongly suggest that WNT8A might be implicated in development of early embryos as well as germ cell tumors through activation of the WNT - beta-catenin - TCF pathway.




Amino Acid Sequence; Blotting, Northern; Cloning, Molecular; Cytoskeletal Proteins/metabolism; DNA Primers/chemistry; DNA, Complementary/genetics; DNA-Binding Proteins/metabolism; Gene Expression; Gene Library; Genomic Library; Humans; Lymphoid Enhancer-Binding Factor 1; Molecular Sequence Data; Neoplasms, Germ Cell and Embryonal/genetics; Neoplasms, Germ Cell and Embryonal/metabolism; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/metabolism; RNA, Messenger/analysis; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Teratocarcinoma/genetics; Teratocarcinoma/metabolism; Trans-Activators; Transcription Factors/metabolism; Wnt Proteins; Wnt2 Protein; Zebrafish Proteins; beta Catenin