GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
TableEdit
PMID:20399743
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
See Help for Help on this wiki. See the documentation for how to use the table editor
| Citation |
Salmanian, S, Najafi, SM, Rafipour, M, Arjomand, MR, Shahheydari, H, Ansari, S, Kashkooli, L, Rasouli, SJ, Jazi, MS and Minaei, T (2010) Regulation of GSK-3beta and beta-Catenin by Galphaq in HEK293T cells. Biochem. Biophys. Res. Commun. 395:577-82 |
|---|---|
| Abstract |
Recent studies have shown that heterotrimeric G proteins are involved in the regulation of the canonical Wnt/beta-Catenin pathway. However, the mechanism(s) behind this involvement is (are) poorly understood. Our previous results have shown that activation of Galphaq in Xenopus oocytes leads to inhibition of GSK-3beta and stabilization of the beta-Catenin protein, suggesting that Galphaq might stabilize beta-Catenin via inhibition of GSK-3beta. In this study, we have observed similar results in HEK293T cells. In these cells optimal activation of endogenous Galphaq by expressing M3-muscarinic acetylcholine receptor (with or without carbachol treatment), or exposing the cells to thrombin led to an increase of 2 to 3-fold in endogenous cytoplasmic beta-Catenin protein levels. In addition, expression of the activated mutant of Galphaq (GalphaqQL) dramatically enhanced accumulation of exogenous beta-Catenin with no effect on beta-catenin (CTNNB1) gene transcription. The Galphaq-mediated cellular accumulation of beta-Catenin was blocked by expression of a minigene encoding a Galphaq specific inhibitory peptide but not by a minigene encoding a Galphas blocking peptide. Also, expression of GalphaqQL led to a significant reduction in GSK-3beta kinase activity, supporting the idea that the positive role of Galphaq signaling in inducing cellular accumulation of beta-Catenin is mediated through inhibition of GSK-3beta. |
| Links |
PubMed Online version:10.1016/j.bbrc.2010.04.087 |
| Keywords |
Cell Line; GTP-Binding Protein alpha Subunits, Gq-G11/genetics; GTP-Binding Protein alpha Subunits, Gq-G11/metabolism; Glycogen Synthase Kinase 3/antagonists & inhibitors; Glycogen Synthase Kinase 3/genetics; Glycogen Synthase Kinase 3/metabolism; Humans; Receptor, Muscarinic M3/agonists; Receptor, Muscarinic M3/biosynthesis; Transcription, Genetic; Wnt Proteins/metabolism; beta Catenin/genetics; beta Catenin/metabolism |
| public |
| Cancel |
