GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search


You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor


Verstreken, P, Ly, CV, Venken, KJ, Koh, TW, Zhou, Y and Bellen, HJ (2005) Synaptic mitochondria are critical for mobilization of reserve pool vesicles at Drosophila neuromuscular junctions. Neuron 47:365-78


In a forward screen for genes affecting neurotransmission in Drosophila, we identified mutations in dynamin-related protein (drp1). DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses. Although resting Ca2+ is elevated at drp1 NMJs, basal synaptic properties are barely affected. However, during intense stimulation, mutants fail to maintain normal neurotransmission. Surprisingly, FM1-43 labeling indicates normal exo- and endocytosis, but a specific inability to mobilize reserve pool vesicles, which is partially rescued by exogenous ATP. Using a variety of drugs, we provide evidence that reserve pool recruitment depends on mitochondrial ATP production downstream of PKA signaling and that mitochondrial ATP limits myosin-propelled mobilization of reserve pool vesicles. Our data suggest a specific role for mitochondria in regulating synaptic strength.


PubMed Online version:10.1016/j.neuron.2005.06.018


Animals; Calcium/metabolism; Cyclic AMP-Dependent Protein Kinases/metabolism; Cytoskeletal Proteins; Drosophila/growth & development; Drosophila/metabolism; Drosophila/physiology; Drosophila/ultrastructure; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Electric Stimulation/methods; Endocytosis; GTP Phosphohydrolases/isolation & purification; GTP Phosphohydrolases/physiology; GTP-Binding Proteins; Larva; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mitochondria/metabolism; Mitochondria/physiology; Mitochondria/ultrastructure; Mutation; Myosin Light Chains/metabolism; Myosins/metabolism; Neuromuscular Junction/metabolism; Neuromuscular Junction/physiology; Neuromuscular Junction/ultrastructure; Neuropeptides/genetics; Neuropeptides/metabolism; Neurotransmitter Agents/metabolism; Presynaptic Terminals/metabolism; Synapses/metabolism; Synapses/physiology; Synaptic Transmission; Synaptic Vesicles/physiology; Tissue Distribution; Vesicular Transport Proteins