GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search


You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor


Hsu, SI, Yang, CM, Sim, KG, Hentschel, DM, O'Leary, E and Bonventre, JV (2001) TRIP-Br: a novel family of PHD zinc finger- and bromodomain-interacting proteins that regulate the transcriptional activity of E2F-1/DP-1. EMBO J. 20:2273-85


We report the isolation of TRIP-Br1, a transcriptional regulator that interacts with the PHD-bromodomain of co-repressors of Krüppel-associated box (KRAB)-mediated repression, KRIP-1(TIF1beta) and TIF1alpha, as well as the co-activator/adaptor p300/CBP. TRIP-Br1 and the related protein TRIP-Br2 possess transactivation domains. Like MDM2, which has a homologous transactivation domain, TRIP-Br proteins functionally contact DP-1, stimulating E2F-1/DP-1 transcriptional activity. KRIP-1 potentiates TRIP-Br protein co-activation of E2F-1/DP-1. TRIP-Br1 is a component of a multiprotein complex containing E2F-1 and DP-1. Co-expression of the retinoblastoma gene product (RB) abolishes baseline E2F-1/DP-1 transcriptional activity as well as TRIP-Br/KRIP-1 co-activation, both of which are restored by the adenovirus E1A oncoprotein. These features suggest that TRIP-Br proteins function at E2F-responsive promoters to integrate signals provided by PHD- and/or bromodomain- containing transcription factors. TRIP-Br1 is identical to the cyclin-dependent kinase 4 (cdk4)-binding protein p34(SEI-1), which renders the activity of cyclin D/cdk4 resistant to the inhibitory effect of p16(INK4a) during late G(1). TRIP-Br1(p34(SEI-1)) is differentially overexpressed during the G(1) and S phases of the cell cycle, consistent with a dual role for TRIP-Br1(p34(SEI-1)) in the regulation of cell cycle progression through sequential effects on the transcriptional activity of E2F-responsive promoters during G(1) and S phases.


PubMed PMC125435 Online version:10.1093/emboj/20.9.2273


Animals; Carrier Proteins; Cell Cycle Proteins; Cell Line; Conserved Sequence; DNA-Binding Proteins/genetics; E2F Transcription Factors; E2F1 Transcription Factor; Gene Expression; Humans; Kruppel-Like Transcription Factors; Mice; Models, Genetic; Molecular Sequence Data; Multigene Family/genetics; Nuclear Proteins/metabolism; Protein Structure, Tertiary/physiology; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins c-mdm2; Repressor Proteins; Retinoblastoma-Binding Protein 1; Sequence Homology, Amino Acid; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factor DP1; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic/physiology; Two-Hybrid System Techniques; Zinc Fingers/physiology