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BPT4:SEGB
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| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status | |
|---|---|---|---|---|---|---|---|---|---|---|---|
|
public |
GO:0004520 |
endodeoxyribonuclease activity |
PMID:18281701 |
ECO:0000314 |
F |
The T4 SegB protein is a site-specific endonuclease that recognizes a 27-bp sequence. 666 bp segB ORF was placed under the control of T7 promoter in pET-15b vector. SegB protein was overproduced in E. coli T7 lac promoter system and purified. The purified protein was incubated with a linear plasmid DNA and products were analyzed of the reaction through gel electrophoresis (Figure 3). SegB cleaved DNA by introducing double-strand breaks in reactions requiring Mg2+, Mn2+, or Ca2+ cations. With Mg2+ and Ca2+ cation, SegB cleaved the DNA at 1 point, creating 2.2 kb and 1.6 kb fragments. In the presence of Mn2+, multiple cleavage sites were present. |
complete | ||||
|
protected |
involved_in |
GO:0090305 |
nucleic acid phosphodiester bond hydrolysis |
GO_REF:0000108 |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0004519 |
P |
Seeded From UniProt |
complete | |
|
protected |
involved_in |
GO:0090305 |
nucleic acid phosphodiester bond hydrolysis |
GO_REF:0000108 |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0004518 |
P |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0004519 |
endonuclease activity |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0255 |
F |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0016787 |
hydrolase activity |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0378 |
F |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0004518 |
nuclease activity |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0540 |
F |
Seeded From UniProt |
complete |
| Cancel |
