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TableEdit
LAMBD:DECO
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| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status | |
|---|---|---|---|---|---|---|---|---|---|---|---|
|
public |
GO:0098021 |
viral capsid, decoration |
PMID:609100 |
ECO:0000315 |
C |
Figure 10 shows that gene D protein, known to be present in the phage head, does not affect head size, suggesting it provides head stability instead. This is verified in Figure 11, which shows that gene D mutants are more susceptible to EDTA inactivation through the rupturing of the phage head. Protein D is likely a decorator protein as it stabilizes the head but is not essential in the gene D mutants isolated (Figure 2). |
complete | ||||
|
public |
GO:0098021 |
viral capsid, decoration |
PMID:25053840 |
ECO:0000314 |
C |
gpD serves as a decoration protein for the capsid and leads to stronger capsid formation. Procapsids were reconstituted in vitro and expanded through 2.5 M urea treatment. Biophysical properties were then assessed through atomic force microscopy. Figure 5 and Table 2 show that addition of gpD leads to an increase in breaking force (0.78 nN from 0.45nN without gpD), and a rescue of the spring constant (0.10 N/m, compared to 0.06 N/m without gpD and the 0.11 N/m of the procapsid). Addition of gpD also reinforces the capsid's stiffness, as shown by the further increase in Young's modulus (E): 0.88 GPa with gpD, 0.57 GPa without gpD, and 0.16 GPa for the procapsid. Based on these measurements, gpD reinforces the capsid, but is not necessary to its formation. |
complete | ||||
|
public |
GO:0019073 |
viral DNA genome packaging |
PMID:18801370 |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Decoration protein gpD contributes to genome packaging in lambda phage. Here, it was evaluated whether gpD is necessary to genome packaging by acting as structural support for the capsid to withstand the high degree of pressure generated. In fig 3A it is shown that addition of gpD of 5uM and above results in a significant increase in the amount of genomic DNA packaged as indicated by the band. 3B is a dose-response curve, showing an increase in genome packaging with increased dose. Fig4 displays the effect of gpD on genome packaging as related to genome length, with no effect on DNA duplexes <37 kb and substantial effect on duplexes >43kb. These results then indicate that gpD reinforces the capsid head such that large DNA duplexes, like the 48kb of lambda phage, can be packaged effectively. |
complete | |||
|
protected |
part_of |
GO:0098021 |
viral capsid, decoration |
PMID:18786402 |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
protected |
part_of |
GO:0019028 |
viral capsid |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0167 |
C |
Seeded From UniProt |
complete | |
|
protected |
part_of |
GO:0019012 |
virion |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0946 |
C |
Seeded From UniProt |
complete | |
|
protected |
part_of |
GO:0030430 |
host cell cytoplasm |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-1035 |
C |
Seeded From UniProt |
complete | |
|
protected |
part_of |
GO:0098021 |
viral capsid, decoration |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-1232 |
C |
Seeded From UniProt |
complete |
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