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SHIF2:D2AJK8
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Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status | |
---|---|---|---|---|---|---|---|---|---|---|---|
public |
GO:0050714 |
positive regulation of protein secretion |
ECO:0000315 |
P |
Fig. 2.A "To characterize the role of spa32, we constructed a S. flexneri 5a strain, MJ321, in which the spa32 gene has been inactivated with a nonpolar cassette... The newly constructed strain was unable to bind CR and was thus white on agar plates at 37°C, suggesting that the spa32 gene product is required for the secretion of Ipa invasins. To test this, the proteins present in the culture supernatants of M90T (wild-type) and MJ321 strains induced by CR were analyzed by SDS-PAGE and immunoblotting. IpaA (70 kDa), IpaB (62 kDa), IpaC (41 kDa), and IpaD (39 kDa) were secreted by the wild-type strain but not by MJ321 (Fig. (Fig.2A).2A)." |
Missing: reference | |||||
public |
GO:0005576 |
extracellular region |
ECO:0000314 |
C |
Figure 3 shows that Spa32 is found in the supernatant of cell culture. |
Missing: reference |
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