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ECOLI:NUOM

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Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

protected

enables

GO:0048039

ubiquinone binding

PMID:21873635

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG11773
PANTHER:PTN000511780

F

Seeded From UniProt

complete

protected

involved_in

GO:0015990

electron transport coupled proton transport

PMID:21873635

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG11773
PANTHER:PTN000511780

P

Seeded From UniProt

complete

protected

involved_in

GO:0009060

aerobic respiration

PMID:21873635

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG11773
PANTHER:PTN000511780

P

Seeded From UniProt

complete

protected

contributes_to

GO:0003954

NADH dehydrogenase activity

PMID:21873635

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG11773
PANTHER:PTN000511780

F

Seeded From UniProt

complete

protected

part_of

GO:0030964

NADH dehydrogenase complex

PMID:7607227

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

protected

part_of

GO:0005886

plasma membrane

PMID:7607227

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

public

Contributes to

GO:0003954

NADH dehydrogenase activity

PMID:17977822

ECO:0000315

F

Figure 4

I used the Contributes to qualifier because, NuoM KO "...lacked a fully assembled NDH-1" showing NuoM is a subunit that is important in the assembly of NDH-1.(1st paragraph under figure 4 in column 2) Without NuoM, the NADH dehydrogenase activity of the complex is inhibited reinforcing the notion that this subunit contributes that activity of the complex. I did not use the NADH (ubiquinone) dehydrogenase activity GO because this paper does not have experimental evidence supporting that.

complete

protected

enables

GO:0048039

ubiquinone binding

PMID:12730198

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

public

GO:0015992

proton transport

PMID:17977822

ECO:0000315

P

Figure 6. Proton gradient was measured using ACMA. They looked at membrane vesicles which had the NDH1 complex containing normal NuoM and mutant NuoM subunits. The vesicles that contained unmutated were able to create a proton gradient where as those that had NuoM knocked out were not suggesting that NuoM is a major factor in the proton translocation process. It is IMP because this was determined by comparing mutant phenotypes of NuoM.

complete

protected

part_of

GO:0045272

plasma membrane respiratory chain complex I

PMID:22063474

ECO:0000315

mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

public

GO:0045838

positive regulation of membrane potential

PMID:17977822

ECO:0000315

P

Figure 5. Membrane vesicles were made containing NDH1 complexs with normal NuoM and mutated NuoM subunits. Membrane potential was measured by changes of oxonol VI. With NuoM, a change in membrane potential was seen but when it was knocked out, there was no change in membrane potential. This suggests NuoM is essential in the complex in order to stimulate membrane potential changes.

complete

protected

part_of

GO:0045272

plasma membrane respiratory chain complex I

PMID:12730198

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

protected

involved_in

GO:0015990

electron transport coupled proton transport

PMID:18590697

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

protected

involved_in

GO:0009060

aerobic respiration

PMID:9495756

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

protected

part_of

GO:0005887

integral component of plasma membrane

PMID:15919996

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

protected

part_of

GO:0005886

plasma membrane

PMID:15919996

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

protected

enables

GO:0008137

NADH dehydrogenase (ubiquinone) activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR003918
InterPro:IPR010227

F

Seeded From UniProt

complete

protected

involved_in

GO:0042773

ATP synthesis coupled electron transport

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR003918
InterPro:IPR010227

P

Seeded From UniProt

complete

protected

involved_in

GO:0055114

oxidation-reduction process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR003918
InterPro:IPR010227

P

Seeded From UniProt

complete

protected

enables

GO:0048038

quinone binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0874

F

Seeded From UniProt

complete

protected

part_of

GO:0016021

integral component of membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0812

C

Seeded From UniProt

complete

protected

part_of

GO:0005886

plasma membrane

GO_REF:0000037
GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1003
UniProtKB-KW:KW-0997
UniProtKB-SubCell:SL-0037

C

Seeded From UniProt

complete

protected

part_of

GO:0016020

membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0472

C

Seeded From UniProt

complete




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