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TableEdit
ECOLI:NUOM
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Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status | |
---|---|---|---|---|---|---|---|---|---|---|---|
protected |
enables |
GO:0048039 |
ubiquinone binding |
PMID:21873635 |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG11773 |
F |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0015990 |
electron transport coupled proton transport |
PMID:21873635 |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG11773 |
P |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0009060 |
aerobic respiration |
PMID:21873635 |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG11773 |
P |
Seeded From UniProt |
complete | |
protected |
contributes_to |
GO:0003954 |
NADH dehydrogenase activity |
PMID:21873635 |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG11773 |
F |
Seeded From UniProt |
complete | |
protected |
part_of |
GO:0030964 |
NADH dehydrogenase complex |
PMID:7607227 |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
protected |
part_of |
GO:0005886 |
plasma membrane |
PMID:7607227 |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
public |
Contributes to |
GO:0003954 |
NADH dehydrogenase activity |
PMID:17977822 |
ECO:0000315 |
F |
Figure 4 I used the Contributes to qualifier because, NuoM KO "...lacked a fully assembled NDH-1" showing NuoM is a subunit that is important in the assembly of NDH-1.(1st paragraph under figure 4 in column 2) Without NuoM, the NADH dehydrogenase activity of the complex is inhibited reinforcing the notion that this subunit contributes that activity of the complex. I did not use the NADH (ubiquinone) dehydrogenase activity GO because this paper does not have experimental evidence supporting that. |
complete | |||
protected |
enables |
GO:0048039 |
ubiquinone binding |
PMID:12730198 |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
public |
GO:0015992 |
proton transport |
PMID:17977822 |
ECO:0000315 |
P |
Figure 6. Proton gradient was measured using ACMA. They looked at membrane vesicles which had the NDH1 complex containing normal NuoM and mutant NuoM subunits. The vesicles that contained unmutated were able to create a proton gradient where as those that had NuoM knocked out were not suggesting that NuoM is a major factor in the proton translocation process. It is IMP because this was determined by comparing mutant phenotypes of NuoM. |
complete | ||||
protected |
part_of |
GO:0045272 |
plasma membrane respiratory chain complex I |
PMID:22063474 |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
public |
GO:0045838 |
positive regulation of membrane potential |
PMID:17977822 |
ECO:0000315 |
P |
Figure 5. Membrane vesicles were made containing NDH1 complexs with normal NuoM and mutated NuoM subunits. Membrane potential was measured by changes of oxonol VI. With NuoM, a change in membrane potential was seen but when it was knocked out, there was no change in membrane potential. This suggests NuoM is essential in the complex in order to stimulate membrane potential changes. |
complete | ||||
protected |
part_of |
GO:0045272 |
plasma membrane respiratory chain complex I |
PMID:12730198 |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
protected |
involved_in |
GO:0015990 |
electron transport coupled proton transport |
PMID:18590697 |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
protected |
involved_in |
GO:0009060 |
aerobic respiration |
PMID:9495756 |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
protected |
part_of |
GO:0005887 |
integral component of plasma membrane |
PMID:15919996 |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
protected |
part_of |
GO:0005886 |
plasma membrane |
PMID:15919996 |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
protected |
enables |
GO:0008137 |
NADH dehydrogenase (ubiquinone) activity |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR003918 |
F |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0042773 |
ATP synthesis coupled electron transport |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR003918 |
P |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0055114 |
oxidation-reduction process |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR003918 |
P |
Seeded From UniProt |
complete | |
protected |
enables |
GO:0048038 |
quinone binding |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0874 |
F |
Seeded From UniProt |
complete | |
protected |
part_of |
GO:0016021 |
integral component of membrane |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0812 |
C |
Seeded From UniProt |
complete | |
protected |
part_of |
GO:0005886 |
plasma membrane |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-1003 |
C |
Seeded From UniProt |
complete | |
protected |
part_of |
GO:0016020 |
membrane |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0472 |
C |
Seeded From UniProt |
complete |
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