GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:18063798
Citation |
Kang, HJ, Coulibaly, F, Clow, F, Proft, T and Baker, EN (2007) Stabilizing isopeptide bonds revealed in gram-positive bacterial pilus structure. Science 318:1625-8 |
---|---|
Abstract |
Many bacterial pathogens have long, slender pili through which they adhere to host cells. The crystal structure of the major pilin subunit from the Gram-positive human pathogen Streptococcus pyogenes at 2.2 angstroms resolution reveals an extended structure comprising two all-beta domains. The molecules associate in columns through the crystal, with each carboxyl terminus adjacent to a conserved lysine of the next molecule. This lysine forms the isopeptide bonds that link the subunits in native pili, validating the relevance of the crystal assembly. Each subunit contains two lysine-asparagine isopeptide bonds generated by an intramolecular reaction, and we find evidence for similar isopeptide bonds in other cell surface proteins of Gram-positive bacteria. The present structure explains the strength and stability of such Gram-positive pili and could facilitate vaccine development. |
Links |
PubMed Online version:10.1126/science.1145806 |
Keywords |
Amino Acid Motifs; Amino Acid Sequence; Asparagine/chemistry; Chemistry, Physical; Crystallography, X-Ray; Fimbriae Proteins/chemistry; Fimbriae, Bacterial/chemistry; Fimbriae, Bacterial/ultrastructure; Hydrogen Bonding; Lysine/chemistry; Models, Molecular; Molecular Sequence Data; Peptides/chemistry; Physicochemical Phenomena; Protein Conformation; Protein Structure, Tertiary; Protein Subunits/chemistry; Streptococcus pyogenes/chemistry; Streptococcus pyogenes/metabolism; Streptococcus pyogenes/ultrastructure |
edit table |
Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0009418: pilus shaft |
ECO:0000315: |
C |
Figure S1. MS/MS spectrum of the peptide with mass-to-charge ratio (m/z) 552.32+ generated from trypsin/AspN double-digest of Spy0128. Mutation of Spy0128 gene leads to degradation of previously protected residues by trypsin |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.