PMID:12490556

Citation	Mukhopadhyay, M, Teufel, A, Yamashita, T, Agulnick, AD, Chen, L, Downs, KM, Schindler, A, Grinberg, A, Huang, SP, Dorward, D and Westphal, H (2003) Functional ablation of the mouse Ldb1 gene results in severe patterning defects during gastrulation. Development 130:495-505
Abstract	The LIM domain-binding protein 1 (Ldb1) is found in multi-protein complexes containing various combinations of LIM-homeodomain, LIM-only, bHLH, GATA and Otx transcription factors. These proteins exert key functions during embryogenesis. Here we show that targeted deletion of the Ldb1 gene in mice results in a pleiotropic phenotype. There is no heart anlage and head structures are truncated anterior to the hindbrain. In about 40% of the mutants, posterior axis duplication is observed. There are also severe defects in mesoderm-derived extraembryonic structures, including the allantois, blood islands of the yolk sack, primordial germ cells and the amnion. Abnormal organizer gene expression during gastrulation may account for the observed axis defects in Ldb1 mutant embryos. The expression of several Wnt inhibitors is curtailed in the mutant, suggesting that Wnt pathways may be involved in axial patterning regulated by Ldb1.
Links	PubMed
Keywords	Animals; Base Sequence; Body Patterning/genetics; Body Patterning/physiology; DNA/genetics; DNA-Binding Proteins/deficiency; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Female; Fetal Heart/embryology; Gastrula/cytology; Gastrula/metabolism; Gene Targeting; LIM Domain Proteins; Male; Mesoderm/cytology; Mice; Mice, Knockout; Oocytes/metabolism; Phenotype; Pregnancy; Proto-Oncogene Proteins/antagonists & inhibitors; Proto-Oncogene Proteins/physiology; RNA, Messenger/genetics; RNA, Messenger/metabolism; Wnt Proteins; Zebrafish Proteins
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