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BRUA2:Q2YNH3

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Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

public

GO:0044177

host cell Golgi apparatus

PMID:23950720

ECO:0000314

C

Figure 3. Confocal micrographs of HeLa cells transfected with Brucella abortus effector protein BspC expressing GFP displayed a golgi apparatus localization. Figure 4. Shows co-localization of BspC with a protein resident in golgi GM130 that confirmed BspC golgi localization.

complete
CACAO 10984

public

GO:0042000

translocation of peptides or proteins into host

PMID:23950720

ECO:0000314

P

Figure 1. TEM1 b-lactamase protein translocation reporter assay. Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_0847 being translocated into J774A.1 host cell (murine cell line). Panel C and D shows representative fluorescence micrographs of protein translocation. Also look Figure S1 and S2.

Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells. Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.

complete
CACAO 10998

public

GO:0034976

response to endoplasmic reticulum stress

PMID:23950720

ECO:0000314

P

Figure S1. Panel B and C shows that, when ectopically expressed in mammalian cells (HEK 293T), BAB1_0847 expression induces significant ER stress to levels similar or higher than a positive control VceC and upregulation of BiP, but not CHOP.

complete
CACAO 11001




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