TableEdit

Liu, S, Cui, H, Li, Q, Zhang, L, Na, Q and Liu, C' (2014) RhoGDI2 Is Expressed in Human Trophoblasts and Involved in Their Migration by Inhibiting the Activation of RAC1. Biol. Reprod. '

The invasion of placental trophoblast cells into the maternal decidua is an essential aspect of placental embedment. The process of placentation bears several striking similarities to tumor cell metastasis. However, trophoblastic migration during implantation and placentation is stringently controlled both in space and time. RhoGDI2 belongs to a family of Rho GDP dissociation inhibitors (RhoGDI), and RhoGDI2 is a metastasis suppressor-gene and a marker of aggressive human cancer. We evaluated whether RhoGDI2 plays a physiological role in embryo implantation, particularly trophoblast migration. The mRNA and protein expression levels of RhoGDI2 were higher in term placentas comparedwith first trimester placentas, as detected by real-time polymerase chain reaction and Western blotting. Immunohistochemical studies indicated that RhoGDI2 localised to the cytotrophoblast layer and extravillous trophoblast in first trimester placentas and was distributed in the syncytiotrophoblast layers of term placentas. Overexpression of RhoGDI2 in HTR-8/SVneo cells was associated with reduced RAC1-GTP levels and inhibited cell migration. Conversely, siRNA-mediated downregulation of RhoGDI2 resulted in a significantly increased RAC1-GTP levels. Altered RhoGDI2 expression had no significant effects on cell proliferation. In conclusion, RhoGDI2 inhibits trophoblast cell migration, and this function may involve suppression of RAC1 activation.

PubMed Online version:10.1095/biolreprod.113.111153