GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
TableEdit
PMID:20956330
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
See Help for Help on this wiki. See the documentation for how to use the table editor
| Citation |
Liscombe, DK, Usera, AR and O'Connor, SE (2010) Homolog of tocopherol C methyltransferases catalyzes N methylation in anticancer alkaloid biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 107:18793-8 |
|---|---|
| Abstract |
Madagascar periwinkle (Catharanthus roseus) is the sole source of the anticancer drugs vinblastine and vincristine, bisindole alkaloids derived from the dimerization of the terpenoid indole alkaloids vindoline and catharanthine. Full elucidation of the biosynthetic pathways of these compounds is a prerequisite for metabolic engineering efforts that will improve production of these costly molecules. However, despite the medical and commercial importance of these natural products, the biosynthetic pathways remain poorly understood. Here we report the identification and characterization of a C. roseus cDNA encoding an S-adenosyl-L-methionine-dependent N methyltransferase that catalyzes a nitrogen methylation involved in vindoline biosynthesis. Recombinant enzyme produced in Escherichia coli is highly substrate specific, displaying a strict requirement for a 2,3-dihydro bond in the aspidosperma skeleton. The corresponding gene transcript is induced in methyl jasmonate-elicited seedlings, along with the other known vindoline biosynthetic transcripts. Intriguingly, this unique N methyltransferase is most similar at the amino acid level to the plastidic γ-tocopherol C methyltransferases of vitamin E biosynthesis, suggesting an evolutionary link between these two functionally disparate methyltransferases. |
| Links |
PubMed PMC2973921 Online version:10.1073/pnas.1009003107 |
| Keywords |
Alkaloids/biosynthesis; Alkaloids/chemistry; Antineoplastic Agents, Phytogenic/biosynthesis; Antineoplastic Agents, Phytogenic/chemistry; Base Sequence; Catharanthus/enzymology; Catharanthus/genetics; Escherichia coli/genetics; Methylation; Methyltransferases/chemistry; Methyltransferases/genetics; Methyltransferases/metabolism; Molecular Sequence Data; Plant Proteins/biosynthesis; Plant Proteins/chemistry; Plant Proteins/genetics; Recombinant Proteins/biosynthesis; Recombinant Proteins/chemistry; Substrate Specificity |
| public |
| Cancel |
