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PMID:22405823
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| Citation |
Kim, SJ, Ryu, MY and Kim, WT (2012) Suppression of Arabidopsis RING-DUF1117 E3 ubiquitin ligases, AtRDUF1 and AtRDUF2, reduces tolerance to ABA-mediated drought stress. Biochem. Biophys. Res. Commun. 420:141-7 |
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| Abstract |
Among approximately 480 RING domain-containing E3 Ub ligases in Arabidopsis, three, At3g46620, At5g59550, and At2g39720, have a domain-of-unknown-function (DUF) 1117 motif in their C-terminal regions. At3g46620 and At5g59550 were identified as homologous ABA- and drought-induced RING-DUF1117 genes and were designated AtRDUF1 and AtRDUF2, respectively. Single and double knock-out mutations of AtRDUFs resulted in hyposensitive phenotypes toward ABA in terms of germination rate and stomatal closure and markedly reduced tolerance to drought stress relative to wild-type plants. These results are discussed in the context that AtRDUF1 and AtRDUF2 play combinatorial, but still distinguishable, roles in ABA-mediated dehydration stress responses. |
| Links |
PubMed Online version:10.1016/j.bbrc.2012.02.131 |
| Keywords |
Amino Acid Motifs; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/physiology; Droughts; Molecular Sequence Data; Mutation; RING Finger Domains; Stress, Physiological/genetics; Ubiquitin-Protein Ligases/genetics; Ubiquitin-Protein Ligases/physiology |
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