GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

TableEdit

Jump to: navigation, search

PMID:10887202

You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor

Citation

Haniu, M, Denis, P, Young, Y, Mendiaz, EA, Fuller, J, Hui, JO, Bennett, BD, Kahn, S, Ross, S, Burgess, T, Katta, V, Rogers, G, Vassar, R and Citron, M (2000) Characterization of Alzheimer's beta -secretase protein BACE. A pepsin family member with unusual properties. J. Biol. Chem. 275:21099-106

Abstract

The cerebral deposition of amyloid beta-peptide is an early and critical feature of Alzheimer's disease. Amyloid beta-peptide is released from the amyloid precursor protein by the sequential action of two proteases, beta-secretase and gamma-secretase, and these proteases are prime targets for therapeutic intervention. We have recently cloned a novel aspartic protease, BACE, with all the known properties of beta-secretase. Here we demonstrate that BACE is an N-glycosylated integral membrane protein that undergoes constitutive N-terminal processing in the Golgi apparatus. We have used a secreted Fc fusion-form of BACE (BACE-IgG) that contains the entire ectodomain for a detailed analysis of posttranslational modifications. This molecule starts at Glu(46) and contains four N-glycosylation sites (Asn(153), Asn(172), Asn(223), and Asn(354)). The six Cys residues in the ectodomain form three intramolecular disulfide linkages (Cys(216)-Cys(420), Cys(278)-Cys(443), and Cys(330)-Cys(380)). Despite the conservation of the active site residues and the 30-37% amino acid homology with known aspartic proteases, the disulfide motif is fundamentally different from that of other aspartic proteases. This difference may affect the substrate specificity of the enzyme. Taken together, both the presence of a transmembrane domain and the unusual disulfide bond structure lead us to conclude that BACE is an atypical pepsin family member.

Links

PubMed Online version:10.1074/jbc.M002095200

Keywords

Amino Acid Sequence; Amyloid Precursor Protein Secretases; Aspartic Acid Endopeptidases/chemistry; Aspartic Acid Endopeptidases/metabolism; Cell Line; Endopeptidases; Glycopeptides/chemistry; Glycoside Hydrolases; Glycosylation; Humans; Models, Molecular; Molecular Sequence Data; Neuraminidase; Pepsin A/metabolism; Peptide Fragments/chemistry; Protein Conformation; RNA Processing, Post-Transcriptional; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

public



Cancel