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CAMJE:ARLY

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Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

public

GO:0004056

argininosuccinate lyase activity

PMID:11683360

ECO:0000314

F

E. coli C600 (WT functional argH),E. coli W3678 (no argH gene), C. jejuni TGH9011 (WT functional argH), and transformed E. coli (C. jejuni TGH9011 argH gene) were examined for ASL activity. E. coli C600, which is wild type with respect to the argH gene, showed a specific activity of 1.1 + 0.17 ,umol of arginine per h per mg of protein. C. jejuni TGH9011 showed a specific activity of 1.3 ± 0.32 ,umol of arginine per h per mg of protein. E. coli W3678 did not possess any significant ASL activity compared with extract-free controls. The transformed strain pARGH1-1 had a specific activity of 5.7 ± 1.2 ,imol of arginine per h per mg of protein. This result confirms that the protein derived from the recombinant plasmid possesses argininosuccinate lyase activity activity.

complete
CACAO 4555

protected

enables

GO:0003824

catalytic activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR008948
InterPro:IPR020557

F

Seeded From UniProt

complete

protected

enables

GO:0004056

argininosuccinate lyase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR009049

F

Seeded From UniProt

complete

protected

involved_in

GO:0042450

arginine biosynthetic process via ornithine

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR009049

P

Seeded From UniProt

complete

protected

enables

GO:0004056

argininosuccinate lyase activity

GO_REF:0000003

ECO:0000501

evidence used in automatic assertion

EC:4.3.2.1

F

Seeded From UniProt

complete

protected

enables

GO:0004056

argininosuccinate lyase activity

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000037443

F

Seeded From UniProt

complete

protected

part_of

GO:0005737

cytoplasm

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000037443

C

Seeded From UniProt

complete

protected

involved_in

GO:0006526

arginine biosynthetic process

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000037443

P

Seeded From UniProt

complete

protected

part_of

GO:0005737

cytoplasm

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0963
UniProtKB-SubCell:SL-0086

C

Seeded From UniProt

complete

protected

involved_in

GO:0006526

arginine biosynthetic process

GO_REF:0000037
GO_REF:0000041

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0055
UniPathway:UPA00068

P

Seeded From UniProt

complete

protected

enables

GO:0016829

lyase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0456

F

Seeded From UniProt

complete

protected

involved_in

GO:0008652

cellular amino acid biosynthetic process

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0028

P

Seeded From UniProt

complete




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