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MYXXD:Q1D7Q3
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| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status | |
|---|---|---|---|---|---|---|---|---|---|---|---|
|
public |
GO:0003677 |
DNA binding |
PMID:PMC1539954 |
ECO:0000314 |
F |
Figure 2 demonstrates an example of one gene promoter region that FruA is able to bind to. E. coli containing a plasmid with the fruA gene was used to produce sufficient quantities of protein after induction with IPTG. 2A demonstrates that FruA was only produced after IPTG induction. The protein was then used in gel-shift assays with P32-labelled DNA, as shown in 2B. The sigma-4400 promoter region, which contains the proposed FruA binding site, forms a band that indicates binding only in the presence of IPTG-induced protein; in other words, only in the presence of FruA. The right side is another gene region from M. xanthus that does not contain the proposed FruA binding site, and acts as a negative control. |
complete | ||||
|
protected |
part_of |
GO:0032993 |
protein-DNA complex |
PMID:16040607 |
ECO:0005634 |
genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion |
RefSeq:NC_008095.1 |
C |
Seeded From UniProt |
complete | |
|
protected |
part_of |
GO:0032993 |
protein-DNA complex |
PMID:16040607 |
ECO:0005631 |
DNAse footprinting evidence used in manual assertion |
RefSeq:NC_008095.1 |
C |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0001216 |
DNA-binding transcription activator activity |
PMID:16040607 |
ECO:0005634 |
genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion |
RefSeq:NC_008095.1 |
F |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0001216 |
DNA-binding transcription activator activity |
PMID:16040607 |
ECO:0005631 |
DNAse footprinting evidence used in manual assertion |
RefSeq:NC_008095.1 |
F |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0000976 |
transcription regulatory region sequence-specific DNA binding |
PMID:16040607 |
ECO:0005634 |
genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion |
RefSeq:NC_008095.1 |
F |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0000976 |
transcription regulatory region sequence-specific DNA binding |
PMID:16040607 |
ECO:0005631 |
DNAse footprinting evidence used in manual assertion |
RefSeq:NC_008095.1 |
F |
Seeded From UniProt |
complete | |
|
protected |
involved_in |
GO:0045893 |
positive regulation of transcription, DNA-templated |
GO_REF:0000108 |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0001216 |
P |
Seeded From UniProt |
complete | |
|
protected |
involved_in |
GO:0045893 |
positive regulation of transcription, DNA-templated |
GO_REF:0000108 |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0001216 |
P |
Seeded From UniProt |
complete | |
|
protected |
involved_in |
GO:0000160 |
phosphorelay signal transduction system |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR001789 |
P |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0003677 |
DNA binding |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR016032 |
F |
Seeded From UniProt |
complete | |
|
protected |
involved_in |
GO:0006355 |
regulation of transcription, DNA-templated |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR000792 |
P |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0003677 |
DNA binding |
GO_REF:0000038 |
ECO:0000323 |
imported automatically asserted information used in automatic assertion |
UniProtKB-KW:KW-0238 |
F |
Seeded From UniProt |
complete |
| Cancel |
