GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
TableEdit
PMID:22105802
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
See Help for Help on this wiki. See the documentation for how to use the table editor
| Citation |
Yin, T, Pan, G, Liu, H, Wu, J, Li, Y, Zhao, Z, Fu, T and Zhou, Y (2012) The chloroplast ribosomal protein L21 gene is essential for plastid development and embryogenesis in Arabidopsis. Planta 235:907-21 |
|---|---|
| Abstract |
Embryogenesis in higher plants is controlled by a complex gene network. Identification and characterization of genes essential for embryogenesis will provide insights into the early events in embryo development. In this study, a novel mutant with aborted seed development (asd) was identified in Arabidopsis. The asd mutant produced about 25% of albino seeds at the early stage of silique development. The segregation of normal and albino seeds was inherited as a single recessive embryo-lethal trait. The gene disrupted in the asd mutant was isolated through map-based cloning. The mutated gene contains a single base change (A to C) in the coding region of RPL21C (At1g35680) that is predicted to encode the chloroplast 50S ribosomal protein L21. Allele test with other two T-DNA insertion lines in RPL21C and a complementation test demonstrated that the mutation in RPL21C was responsible for the asd phenotype. RPL21C exhibits higher expression in leaves and flowers compared with expression levels in roots and developing seeds. The RPL21C-GFP fusion protein was localized in chloroplasts. Cytological observations showed that the asd embryo development was arrested at the globular stage. There were no plastids with normal thylakoids and as a result no normal chloroplasts formed in mutant cells, indicating an indispensable role of the ASD gene in chloroplasts biogenesis. Our studies suggest that the chloroplast ribosomal protein L21 gene is required for chloroplast development and embryogenesis in Arabidopsis. |
| Links |
PubMed Online version:10.1007/s00425-011-1547-0 |
| Keywords |
Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/metabolism; Chloroplasts/genetics; Gene Expression Regulation, Plant; Genes, Plant/genetics; Genetic Variation; Genotype; Phenotype; Pigmentation; Plant Growth Regulators/metabolism; Plants, Genetically Modified; Plastids/physiology; Point Mutation; Ribosomal Proteins/metabolism; Seeds/cytology; Seeds/growth & development |
| public |
| Cancel |
