GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

TableEdit

Jump to: navigation, search

PMID:21390326

You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor

Citation

Burgess, K, Xu, T, Brown, R, Han, B and Welle, S (2011) Effect of myostatin depletion on weight gain, hyperglycemia, and hepatic steatosis during five months of high-fat feeding in mice. PLoS ONE 6:e17090

Abstract

The marked hypermuscularity in mice with constitutive myostatin deficiency reduces fat accumulation and hyperglycemia induced by high-fat feeding, but it is unclear whether the smaller increase in muscle mass caused by postdevelopmental loss of myostatin activity has beneficial metabolic effects during high-fat feeding. We therefore examined how postdevelopmental myostatin knockout influenced effects of high-fat feeding. Male mice with ubiquitous expression of tamoxifen-inducible Cre recombinase were fed tamoxifen for 2 weeks at 4 months of age. This depleted myostatin in mice with floxed myostatin genes, but not in control mice with normal myostatin genes. Some mice were fed a high-fat diet (60% of energy) for 22 weeks, starting 2 weeks after cessation of tamoxifen feeding. Myostatin depletion increased skeletal muscle mass ∼30%. Hypermuscular mice had ∼50% less weight gain than control mice over the first 8 weeks of high-fat feeding. During the subsequent 3 months of high-fat feeding, additional weight gain was similar in control and myostatin-deficient mice. After 5 months of high-fat feeding, the mass of epididymal and retroperitoneal fat pads was similar in control and myostatin-deficient mice even though myostatin depletion reduced the weight gain attributable to the high-fat diet (mean weight with high-fat diet minus mean weight with low-fat diet: 19.9 g in control mice, 14.1 g in myostatin-deficient mice). Myostatin depletion did not alter fasting blood glucose levels after 3 or 5 months of high-fat feeding, but reduced glucose levels measured 90 min after intraperitoneal glucose injection. Myostatin depletion also attenuated hepatic steatosis and accumulation of fat in muscle tissue. We conclude that blocking myostatin signaling after maturity can attenuate some of the adverse effects of a high-fat diet.

Links

PubMed PMC3044753 Online version:10.1371/journal.pone.0017090

Keywords

Animals; Blood Glucose/analysis; Blood Glucose/drug effects; Blood Glucose/metabolism; Diet, Atherogenic; Dietary Fats/adverse effects; Fatty Liver/blood; Fatty Liver/complications; Fatty Liver/etiology; Fatty Liver/genetics; Glucose/administration & dosage; Glucose/pharmacology; Hyperglycemia/blood; Hyperglycemia/chemically induced; Hyperglycemia/complications; Hyperglycemia/genetics; Injections, Intraperitoneal; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; Myostatin/genetics; Myostatin/metabolism; Myostatin/physiology; Time Factors; Weight Gain/drug effects; Weight Gain/genetics; Weight Gain/physiology

public



Cancel