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TableEdit
LACCA:PTLCB
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| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status | |
|---|---|---|---|---|---|---|---|---|---|---|---|
|
public |
GO:0016310 |
phosphorylation |
PMID:2125053 |
ECO:0000315 |
P |
Fig. 5 Lanes 1-4 indicate phosphorylation activity. |
complete | ||||
|
public |
GO:0046835 |
carbohydrate phosphorylation |
PMID:2125053 |
ECO:0000315 |
P |
As seen in figure 7, lanes 1 through 6, when each cysteine residue was replaced in turn by serine, lactose phosphorylation was found in mutants in which cysteine residues 31, 137, 152, 235, 316, or 349 had been replaced. As seen in figure 7, lane 7, no phosphorylation of the substrate occurred when Cys483 was replaced with a serine. As seen in figure 7, lanes 7 through 9, to confirm that this effect was not due to an undetected secondary mutation, a total of five independently obtained Cys483+Ser483 mutants, from two different experiments, were assayed. It was found in all cases that these mutants did not phosphorylate lactose. As seen in figure 7, lanes 10 through 11, when Cys483 was replaced with histidine, an amino acid found to be involved in all other phosphoryltransfer reactions of the PTS, or tyrosine, no lactose phosphorylation was observed. The loss of phosphorylating activity appeared to be specifically due to the exchange of Cys483 and not due to disruption of the tertiary structure of the protein. Cys483 is the active center phosphorylated amino acid residue in EIIlacL.casei. |
complete | ||||
|
public |
GO:0005886 |
plasma membrane |
PMID:PMC2753894 |
ECO:0000315 |
C |
Figure 5: Presence of intact peptidoglycan |
complete | ||||
|
protected |
involved_in |
GO:0046835 |
carbohydrate phosphorylation |
PMID:2125053 |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
protected |
involved_in |
GO:0016310 |
phosphorylation |
PMID:2125053 |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
protected |
involved_in |
GO:0015767 |
lactose transport |
GO_REF:0000108 |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0022869 |
P |
Seeded From UniProt |
complete | |
|
protected |
involved_in |
GO:0034219 |
carbohydrate transmembrane transport |
GO_REF:0000108 |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0008982 |
P |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR003352 |
F |
Seeded From UniProt |
complete | |
|
protected |
involved_in |
GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR003352 |
P |
Seeded From UniProt |
complete | |
|
protected |
part_of |
GO:0016020 |
membrane |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR003352 |
C |
Seeded From UniProt |
complete | |
|
protected |
part_of |
GO:0016021 |
integral component of membrane |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR004501 |
C |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0022869 |
protein-N(PI)-phosphohistidine-lactose phosphotransferase system transporter activity |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR004801 |
F |
Seeded From UniProt |
complete | |
|
protected |
part_of |
GO:0016021 |
integral component of membrane |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0812 |
C |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0016301 |
kinase activity |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0418 |
F |
Seeded From UniProt |
complete | |
|
protected |
involved_in |
GO:0016310 |
phosphorylation |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0418 |
P |
Seeded From UniProt |
complete | |
|
protected |
part_of |
GO:0016020 |
membrane |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0472 |
C |
Seeded From UniProt |
complete | |
|
protected |
involved_in |
GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0598 |
P |
Seeded From UniProt |
complete | |
|
protected |
involved_in |
GO:0008643 |
carbohydrate transport |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0762 |
P |
Seeded From UniProt |
complete | |
|
protected |
enables |
GO:0016740 |
transferase activity |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0808 |
F |
Seeded From UniProt |
complete | |
|
protected |
part_of |
GO:0005886 |
plasma membrane |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-1003 |
C |
Seeded From UniProt |
complete |
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