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PMID:21148004
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| Citation |
Chen, B, Ge, Q, Xia, XM, Liu, P, Wang, SJ, Zhan, H, Eipper, BA and Wang, ZW (2010) A novel auxiliary subunit critical to BK channel function in Caenorhabditis elegans. J. Neurosci. 30:16651-61 |
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| Abstract |
The BK channel is a Ca²+- and voltage-gated potassium channel with many important physiological functions. To identify proteins important to its function in vivo, we screened for Caenorhabditis elegans mutants that suppressed a lethargic phenotype caused by expressing a gain-of-function (gf) isoform of the BK channel α-subunit SLO-1. BKIP-1 (for BK channel interacting protein), a small peptide with no significant homology to any previously characterized molecules, was thus identified. BKIP-1 and SLO-1 showed similar expression and subcellular localization patterns and appeared to interact physically through discrete domains. bkip-1 loss-of-function (lf) mutants phenocopied slo-1(lf) mutants in behavior and synaptic transmission and suppressed the lethargy, egg-laying defect, and deficient neurotransmitter release caused by SLO-1(gf). In heterologous expression systems, BKIP-1 decreased the activation rate and shifted the conductance-voltage relationship of SLO-1 in a Ca²+-dependent manner and increased SLO-1 surface expression. Thus, BKIP-1 is a novel auxiliary subunit critical to SLO-1 function in vivo. |
| Links |
PubMed PMC3076056 Online version:10.1523/JNEUROSCI.3211-10.2010 |
| Keywords |
Amino Acid Sequence; Animals; Animals, Genetically Modified; Behavior, Animal; Biotinylation/methods; Caenorhabditis elegans; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/physiology; Excitatory Postsynaptic Potentials/genetics; Humans; Immunoprecipitation/methods; Large-Conductance Calcium-Activated Potassium Channels/chemistry; Large-Conductance Calcium-Activated Potassium Channels/genetics; Large-Conductance Calcium-Activated Potassium Channels/physiology; Locomotion/genetics; Luminescent Proteins/genetics; Membrane Potentials/drug effects; Membrane Potentials/genetics; Models, Molecular; Molecular Sequence Data; Mutation/genetics; Neurons/physiology; Oocytes; Patch-Clamp Techniques/methods; Protein Subunits/genetics; Protein Subunits/physiology; Reproductive Behavior/physiology; Synaptic Transmission/genetics; Xenopus |
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