TableEdit

Pappas, CT, Krieg, PA and Gregorio, CC (2010) Nebulin regulates actin filament lengths by a stabilization mechanism. J. Cell Biol. 189:859-70

Efficient muscle contraction requires regulation of actin filament lengths. In one highly cited model, the giant protein nebulin has been proposed to function as a molecular ruler specifying filament lengths. We directly challenged this hypothesis by constructing a unique, small version of nebulin (mini-nebulin). When endogenous nebulin was replaced with mini-nebulin in skeletal myocytes, thin filaments extended beyond the end of mini-nebulin, an observation which is inconsistent with a strict ruler function. However, under conditions that promote actin filament depolymerization, filaments associated with mini-nebulin were remarkably maintained at lengths either matching or longer than mini-nebulin. This indicates that mini-nebulin is able to stabilize portions of the filament it has no contact with. Knockdown of nebulin also resulted in more dynamic populations of thin filament components, whereas expression of mini-nebulin decreased the dynamics at both filament ends (i.e., recovered loss of endogenous nebulin). Thus, nebulin regulates thin filament architecture by a mechanism that includes stabilizing the filaments and preventing actin depolymerization.

PubMed PMC2878950 Online version:10.1083/jcb.201001043

Actin Cytoskeleton/drug effects; Actin Cytoskeleton/metabolism; Actins/metabolism; Animals; Bicyclo Compounds, Heterocyclic/pharmacology; Chickens; Humans; Muscle Fibers, Skeletal/drug effects; Muscle Fibers, Skeletal/metabolism; Muscle Proteins/genetics; Muscle Proteins/metabolism; Peptide Fragments/genetics; Peptide Fragments/metabolism; RNA, Small Interfering/genetics; Thiazolidines/pharmacology; Transfection; Tropomodulin/metabolism