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Ruotsalainen, H, Sipilä, L, Vapola, M, Sormunen, R, Salo, AM, Uitto, L, Mercer, DK, Robins, SP, Risteli, M, Aszodi, A, Fässler, R and Myllylä, R (2006) Glycosylation catalyzed by lysyl hydroxylase 3 is essential for basement membranes. J. Cell. Sci. 119:625-35

Lysyl hydroxylase 3 (LH3) is a multifunctional enzyme possessing lysyl hydroxylase (LH), hydroxylysyl galactosyltransferase (GT) and galactosylhydroxylysyl glucosyltransferase (GGT) activities in vitro. To investigate the in vivo importance of LH3-catalyzed lysine hydroxylation and hydroxylysine-linked glycosylations, three different LH3-manipulated mouse lines were generated. Mice with a mutation that blocked only the LH activity of LH3 developed normally, but showed defects in the structure of the basement membrane and in collagen fibril organization in newborn skin and lung. Analysis of a hypomorphic LH3 mouse line with the same mutation, however, demonstrated that the reduction of the GGT activity of LH3 disrupts the localization of type IV collagen, and thus the formation of basement membranes during mouse embryogenesis leading to lethality at embryonic day (E) 9.5-14.5. Strikingly, survival of hypomorphic embryos and the formation of the basement membrane were directly correlated with the level of GGT activity. In addition, an LH3-knockout mouse lacked GGT activity leading to lethality at E9.5. The results confirm that LH3 has LH and GGT activities in vivo, LH3 is the main molecule responsible for GGT activity and that the GGT activity, not the LH activity of LH3, is essential for the formation of the basement membrane. Together our results demonstrate for the first time the importance of hydroxylysine-linked glycosylation for collagens.

PubMed Online version:10.1242/jcs.02780

Animals; Basement Membrane/enzymology; Catalysis; Collagen/chemistry; Collagen/metabolism; Galactosyltransferases/metabolism; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Glucosyltransferases/metabolism; Glycosylation; Hydroxylysine/metabolism; Mice; Mice, Knockout; Mutation; Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase/chemistry; Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase/genetics; Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase/metabolism; Substrate Specificity