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PMID:16682526

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Citation

Madrid, AS, Mancuso, J, Cande, WZ and Weis, K (2006) The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function. J. Cell Biol. 173:361-71

Abstract

The nuclear pore complex (NPC) is a large channel that spans the two lipid bilayers of the nuclear envelope and mediates transport events between the cytoplasm and the nucleus. Only a few NPC components are transmembrane proteins, and the role of these proteins in NPC function and assembly remains poorly understood. We investigate the function of the three integral membrane nucleoporins, which are Ndc1p, Pom152p, and Pom34p, in NPC assembly and transport in Saccharomyces cerevisiae. We find that Ndc1p is important for the correct localization of nuclear transport cargoes and of components of the NPC. However, the role of Ndc1p in NPC assembly is partially redundant with Pom152p, as cells lacking both of these proteins show enhanced NPC disruption. Electron microscopy studies reveal that the absence of Ndc1p and Pom152p results in aberrant pores that have enlarged diameters and lack proteinaceous material, leading to an increased diffusion between the cytoplasm and the nucleus.

Links

PubMed PMC2063837 Online version:10.1083/jcb.200506199

Keywords

Active Transport, Cell Nucleus; Cell Division/genetics; Cell Division/physiology; Cell Nucleus/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Diffusion; Membrane Glycoproteins/genetics; Membrane Glycoproteins/physiology; Microscopy, Electron, Transmission; Mitotic Spindle Apparatus/genetics; Mitotic Spindle Apparatus/physiology; Mutation; Nuclear Localization Signals/genetics; Nuclear Localization Signals/metabolism; Nuclear Pore/metabolism; Nuclear Pore/physiology; Nuclear Pore/ultrastructure; Nuclear Pore Complex Proteins/genetics; Nuclear Pore Complex Proteins/metabolism; Nuclear Pore Complex Proteins/physiology; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Nuclear Proteins/physiology; RNA Transport; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology; Transcription Factors/genetics; Transcription Factors/metabolism

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