GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

TableEdit

Jump to: navigation, search

PMID:16219799

You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor

Citation

Hamaguchi, I, Morisada, T, Azuma, M, Murakami, K, Kuramitsu, M, Mizukami, T, Ohbo, K, Yamaguchi, K, Oike, Y, Dumont, DJ and Suda, T (2006) Loss of Tie2 receptor compromises embryonic stem cell-derived endothelial but not hematopoietic cell survival. Blood 107:1207-13

Abstract

Tie2 is a receptor-type tyrosine kinase expressed on hematopoietic stem cells and endothelial cells. We used cultured embryonic stem (ES) cells to determine the function of Tie2 during early vascular development and hematopoiesis. Upon differentiation, the ES cell-derived Tie2+ Flk1+ fraction was enriched for hematopoietic and endothelial progenitor cells. To investigate lymphatic differentiation, we used a monoclonal antibody against LYVE-1 and found that LYVE-1+ cells derived from Tie2+ Flk1+ cells possessed various characteristics of lymphatic endothelial cells. To determine whether Tie2 played a role in this process, we analyzed differentiation of Tie2-/- ES cells. Although the initial numbers of LYVE-1+ and PECAM-1+ cells derived from Tie2-/- cells did not vary significantly, the number of both decreased dramatically upon extended culturing. Such decreases were rescued by treatment with a caspase inhibitor, suggesting that reductions were due to apoptosis as a consequence of a lack of Tie2 signaling. Interestingly, Tie2-/- ES cells did not show measurable defects in development of the hematopoietic system, suggesting that Tie2 is not essential for hematopoietic cell development.

Links

PubMed PMC1895914 Online version:10.1182/blood-2005-05-1823

Keywords

Animals; Antigens, CD31/metabolism; Cell Differentiation/physiology; Cell Survival/physiology; Cells, Cultured; Embryo, Mammalian/cytology; Embryo, Mammalian/physiology; Endothelial Cells/cytology; Endothelial Cells/physiology; Glycoproteins/metabolism; Hematopoiesis/physiology; Hematopoietic Stem Cells/cytology; Hematopoietic Stem Cells/physiology; Mice; Neovascularization, Physiologic/physiology; Receptor, TIE-2/metabolism; Signal Transduction/physiology

public



Cancel