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PMID:20170636

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Citation

Qian, Y, West, CM and Kornfeld, S (2010) UDP-GlcNAc:Glycoprotein N-acetylglucosamine-1-phosphotransferase mediates the initial step in the formation of the methylphosphomannosyl residues on the high mannose oligosaccharides of Dictyostelium discoideum glycoproteins. Biochem. Biophys. Res. Commun. 393:678-81

Abstract

The Dictyostelium discoideum gene gpt1 encodes a protein XP_638036 with sequence similarity to the alpha/beta subunits of mammalian UDP-GlcNAc:Glycoprotein N-acetylglucosamine-1-phosphotransferase. We now demonstrate that extracts of D. discoideum clones with mutations in this gene transfer GlcNAc-P from UDP-GlcNAc to mannose residues at less than 5% the wild type value. Further, the lysosomal hydrolases of these mutant clones fail to bind to a cation-independent mannose 6-phosphate receptor affinity column, indicating a lack of methylphosphomannosyl residues on the high mannose oligosaccharides of these proteins. We conclude that the gpt1 gene product catalyzes the initial step in the formation of methylphosphomannosyl residues on D. discoideum lysosomal hydrolases.

Links

PubMed PMC2846398 Online version:10.1016/j.bbrc.2010.02.055

Keywords

Catalysis; Dictyostelium/enzymology; Dictyostelium/genetics; Glycoproteins/metabolism; Mannose/metabolism; Oligosaccharides/metabolism; Transferases (Other Substituted Phosphate Groups)/genetics; Transferases (Other Substituted Phosphate Groups)/metabolism

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