GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

TableEdit

Jump to: navigation, search

PMID:18653886

You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor

Citation

Guang, S, Bochner, AF, Pavelec, DM, Burkhart, KB, Harding, S, Lachowiec, J and Kennedy, S (2008) An Argonaute transports siRNAs from the cytoplasm to the nucleus. Science 321:537-41

Abstract

Ribonucleoprotein complexes consisting of Argonaute-like proteins and small regulatory RNAs function in a wide range of biological processes. Many of these small regulatory RNAs are predicted to act, at least in part, within the nucleus. We conducted a genetic screen to identify factors essential for RNA interference (RNAi) in nuclei of Caenorhabditis elegans and identified the Argonaute protein NRDE-3. In the absence of small interfering RNAs (siRNAs), NRDE-3 resides in the cytoplasm. NRDE-3 binds siRNAs generated by RNA-dependent RNA polymerases acting on messenger RNA templates in the cytoplasm and redistributes to the nucleus. Nuclear redistribution of NRDE-3 requires a functional nuclear localization signal, is required for nuclear RNAi, and results in NRDE-3 association with nuclear-localized nascent transcripts. Thus, specific Argonaute proteins can transport specific classes of small regulatory RNAs to distinct cellular compartments to regulate gene expression.

Links

PubMed PMC2771369 Online version:10.1126/science.1157647

Keywords

Active Transport, Cell Nucleus; Animals; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/growth & development; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/chemistry; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Cell Nucleus/metabolism; Cytoplasm/metabolism; Genes, Helminth; Mutation; Nuclear Localization Signals; Protein Structure, Tertiary; RNA Interference; RNA Precursors/genetics; RNA Precursors/metabolism; RNA Replicase/metabolism; RNA, Double-Stranded/chemistry; RNA, Double-Stranded/genetics; RNA, Double-Stranded/metabolism; RNA, Helminth/chemistry; RNA, Helminth/genetics; RNA, Helminth/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Small Interfering/chemistry; RNA, Small Interfering/genetics; RNA, Small Interfering/metabolism; RNA-Binding Proteins/chemistry; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Up-Regulation

public



Cancel