GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
TableEdit
PMID:15141165
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
See Help for Help on this wiki. See the documentation for how to use the table editor
Citation |
Schröder, M, Clark, R, Liu, CY and Kaufman, RJ (2004) The unfolded protein response represses differentiation through the RPD3-SIN3 histone deacetylase. EMBO J. 23:2281-92 |
---|---|
Abstract |
In Saccharomyces cerevisiae, splicing of HAC1 mRNA is initiated in response to the accumulation of unfolded proteins in the endoplasmic reticulum by the transmembrane kinase-endoribonuclease Ire1p. Spliced Hac1p (Hac1ip) is a negative regulator of differentiation responses to nitrogen starvation, pseudohyphal growth, and meiosis. Here we show that the RPD3-SIN3 histone deacetylase complex (HDAC), its catalytic activity, recruitment of the HDAC to the promoters of early meiotic genes (EMGs) by Ume6p, and the Ume6p DNA-binding site URS1 in the promoters of EMGs are required for nitrogen-mediated negative regulation of EMGs and meiosis by Hac1ip. Co-immunoprecipitation experiments demonstrated that Hac1ip can interact with the HDAC in vivo. Systematic analysis of double deletion strains revealed that HAC1 is a peripheral component of the HDAC. In summary, nitrogen-induced synthesis of Hac1ip and association of Hac1ip with the HDAC are physiological events in the regulation of EMGs by nutrients. These data also define for the first time a gene class that is under negative control by the UPR, and provide the framework for a novel mechanism through which bZIP proteins repress transcription. |
Links |
PubMed PMC419911 Online version:10.1038/sj.emboj.7600233 |
Keywords |
Binding Sites; Cell Differentiation; DNA-Binding Proteins/metabolism; Fungal Proteins/metabolism; Gene Deletion; Gene Expression Regulation, Fungal; Genes, Fungal; Histone Deacetylases/metabolism; Meiosis; Membrane Glycoproteins/metabolism; Models, Biological; Nitrogen/metabolism; Precipitin Tests; Promoter Regions, Genetic; Protein Folding; Protein-Serine-Threonine Kinases/metabolism; RNA Splicing; Repressor Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Sin3 Histone Deacetylase and Corepressor Complex; Transcription Factors/metabolism |
public |
Cancel |