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PMID:12121891
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| Citation |
Arima, K, Hines, ER, Kiela, PR, Drees, JB, Collins, JF and Ghishan, FK (2002) Glucocorticoid regulation and glycosylation of mouse intestinal type IIb Na-P(i) cotransporter during ontogeny. Am. J. Physiol. Gastrointest. Liver Physiol. 283:G426-34 |
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| Abstract |
We sought to characterize expression of an apically expressed intestinal Na-P(i) cotransporter (Na-P(i)-IIb) during mouse ontogeny and to assess the effects of methylprednisolone (MP) treatment. In control mice, Na-P(i) uptake by intestinal brush-border membrane vesicles was highest at 14 days of age, lower at 21 days, and further reduced at 8 wk and 8-9 mo of age. Na-P(i)-IIb mRNA and immunoreactive protein levels in 14-day-old animals were markedly higher than in older groups. MP treatment significantly decreased Na-P(i) uptake and Na-P(i)-IIb mRNA and protein expression in 14-day-old mice. Additionally, the size of the protein was smaller in 14-day-old mice. Deglycosylation of protein from 14-day-old and 8-wk-old animals with peptide N-glycosidase reduced the molecular weight to the predicted size. We conclude that intestinal Na-P(i) uptake and Na-P(i)-IIb expression are highest at 14 days and decrease with age. Furthermore, MP treatment reduced intestinal Na-P(i) uptake approximately threefold in 14-day-old mice and this reduction correlates with reduced Na-P(i)-IIb mRNA and protein expression. We also demonstrate that Na-P(i)-IIb is an N-linked glycoprotein and that glycosylation is age dependent. |
| Links |
PubMed Online version:10.1152/ajpgi.00319.2001 |
| Keywords |
Aging/physiology; Amino Acid Sequence/genetics; Animals; Animals, Newborn/growth & development; Animals, Newborn/physiology; Animals, Suckling/physiology; Blotting, Northern; Glycoside Hydrolases/pharmacology; Glycosylation; Immune Sera/immunology; Intestines/metabolism; Male; Methylprednisolone/pharmacology; Mice; Mice, Inbred C57BL; Microvilli/metabolism; Molecular Sequence Data; Protein Isoforms/immunology; Protein Isoforms/metabolism; Sodium-Phosphate Cotransporter Proteins; Sodium-Phosphate Cotransporter Proteins, Type IIb; Symporters/genetics; Symporters/immunology; Symporters/metabolism; Weaning |
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