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PMID:17426032

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Citation

Rasbery, JM, Shan, H, LeClair, RJ, Norman, M, Matsuda, SP and Bartel, B (2007) Arabidopsis thaliana squalene epoxidase 1 is essential for root and seed development. J. Biol. Chem. 282:17002-13

Abstract

Squalene epoxidase converts squalene into oxidosqualene, the precursor of all known angiosperm cyclic triterpenoids, which include membrane sterols, brassinosteroid phytohormones, and non-steroidal triterpenoids. In this work, we have identified six putative Arabidopsis squalene epoxidase (SQE) enzymes and used heterologous expression in yeast to demonstrate that three of these enzymes, SQE1, SQE2, and SQE3, can epoxidize squalene. We isolated and characterized Arabidopsis sqe1 mutants and discovered severe developmental defects, including reduced root and hypocotyl elongation. Adult sqe1-3 and sqe1-4 plants have diminished stature and produce inviable seeds. The sqe1-3 mutant accumulates squalene, consistent with a block in the triterpenoid biosynthetic pathway. Therefore, SQE1 function is necessary for normal plant development, and the five SQE-like genes remaining in this mutant are not fully redundant with SQE1.

Links

PubMed Online version:10.1074/jbc.M611831200

Keywords

Amino Acid Sequence; Arabidopsis/embryology; Arabidopsis/enzymology; Arabidopsis/growth & development; Base Sequence; DNA Primers; Genes, Plant; Molecular Sequence Data; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Seeds/growth & development; Sequence Homology, Amino Acid; Squalene Monooxygenase/chemistry; Squalene Monooxygenase/genetics; Squalene Monooxygenase/metabolism

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