GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
TableEdit
PMID:14595688
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
See Help for Help on this wiki. See the documentation for how to use the table editor
| Citation |
Borderies, G, Jamet, E, Lafitte, C, Rossignol, M, Jauneau, A, Boudart, G, Monsarrat, B, Esquerré-Tugayé, MT, Boudet, A and Pont-Lezica, R (2003) Proteomics of loosely bound cell wall proteins of Arabidopsis thaliana cell suspension cultures: a critical analysis. Electrophoresis 24:3421-32 |
|---|---|
| Abstract |
The complete sequencing of the Arabidopsis thaliana genome allows the use of the recently developed mass spectrometry techniques to identify the cell wall proteins (CWPs). Most proteomic approaches depend on the quality of sample preparation. Extraction of CWPs is particularly complex since the proteins may be free in the apoplast or are embedded in a polysaccharide matrix where they are retained by Van der Waals interactions, hydrogen bonds, hydrophobic or ionic interactions, or cross-linked by covalent bonds. Specific and sequential extraction procedures thus need to be developed. We report on the sequential extraction of loosely bound CWPs from living A. thaliana cells in culture. Different salts and chelating agents were used for releasing the proteins from the wall. Their effects on the extraction of CWPs and on the integrity of the plasma membrane were evaluated. Bioinformatic software was used to identify proteins and to predict their sub-cellular localization. The obtained data show that the plasma membrane of cells in culture was easily damaged by some steps of the extraction procedure, leading to the release of increasing amounts of intracellular proteins. Nevertheless, we identified fifty CWPs among which thirteen were new proteins for the cell wall. In addition, 76% of these CWPs were basic proteins not resolved in two-dimensional (2-D) gel electrophoresis. The existence of two hypothetical proteins was confirmed. The structure of three proteins could be confirmed using mass spectrometry data. |
| Links |
PubMed Online version:10.1002/elps.200305608 |
| Keywords |
Arabidopsis/chemistry; Arabidopsis/cytology; Arabidopsis Proteins/analysis; Arabidopsis Proteins/classification; Arabidopsis Proteins/isolation & purification; Cell Fractionation; Cell Wall/chemistry; Computational Biology; Electrophoresis, Gel, Two-Dimensional; Mass Spectrometry; Plant Extracts/analysis; Plant Proteins/analysis; Plant Proteins/classification; Plant Proteins/isolation & purification; Proteomics/methods |
| public |
| Cancel |
