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PMID:10637294
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| Citation |
Kerscher, O, Sepuri, NB and Jensen, RE (2000) Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane. Mol. Biol. Cell 11:103-16 |
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| Abstract |
The mitochondrial inner membrane contains two separate translocons: one required for the translocation of matrix-targeted proteins (the Tim23p-Tim17p complex) and one for the insertion of polytopic proteins into the mitochondrial inner membrane (the Tim54p-Tim22p complex). To identify new members of the Tim54p-Tim22p complex, we screened for high-copy suppressors of the temperature-sensitive tim54-1 mutant. We identified a new gene, TIM18, that encodes an integral protein of the inner membrane. The following genetic and biochemical observations suggest that the Tim18 protein is part of the Tim54p-Tim22p complex in the inner membrane: multiple copies of TIM18 suppress the tim54-1 growth defect; the tim18::HIS3 disruption is synthetically lethal with tim54-1; Tim54p and Tim22p can be coimmune precipitated with the Tim18 protein; and Tim18p, along with Tim54p and Tim22p, is detected in an approximately 300-kDa complex after blue native electrophoresis. We propose that Tim18p is a new component of the Tim54p-Tim22p machinery that facilitates insertion of polytopic proteins into the mitochondrial inner membrane. |
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| Keywords |
Amino Acid Sequence; Animals; Antiporters; Base Sequence; Binding Sites; Biological Transport; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Division; Fungal Proteins/genetics; Fungal Proteins/metabolism; Hydro-Lyases/genetics; Hydro-Lyases/metabolism; Intracellular Membranes/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Membrane Transport Proteins; Mitochondria/metabolism; Mitochondrial Membrane Transport Proteins; Molecular Sequence Data; Rabbits; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins |
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