GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
TableEdit
PMID:11511099
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
See Help for Help on this wiki. See the documentation for how to use the table editor
| Citation |
Melis, E, Moons, L, De Mol, M, Herbert, JM, Mackman, N, Collen, D, Carmeliet, P and Dewerchin, M (2001) Targeted deletion of the cytosolic domain of tissue factor in mice does not affect development. Biochem. Biophys. Res. Commun. 286:580-6 |
|---|---|
| Abstract |
The role of the cytosolic domain of tissue factor (TF) in signal transduction and gene regulation was studied in mice with a targeted deletion of the 18 carboxy-terminal intracellular amino acids. This deletion was introduced in exon 6 along with a floxed neo(R) selection cassette in intron 5 using homologous recombination in embryonic stem cells. Removal of the floxed neo(R) cassette by in vivo Cre-mediated loxP recombination yielded TF(+/deltaCT) and TF(deltaCT/deltaCT) mice. In contrast to TF(-/-) mice, TF(+/deltaCT) and TF(deltaCT/deltaCT) mice displayed normal embryonic development, survival, fertility, and blood coagulation. Factor VIIa or factor Xa stimulation produced similar p44/42 MAPK activation in TF(+/+) and TF(deltaCT/deltaCT) fibroblasts. These data, based on expression of a TF(deltaCT) molecule from the endogenous TF locus, provide conclusive proof that the cytosolic domain of TF is not essential for signal transduction in embryogenesis and in physiological postnatal processes. |
| Links |
PubMed Online version:10.1006/bbrc.2001.5425 |
| Keywords |
Animals; Cells, Cultured; Embryo, Mammalian/blood supply; Embryonic and Fetal Development; Factor VIIa/pharmacology; Factor Xa/pharmacology; Fibroblasts/drug effects; Fibroblasts/metabolism; Gene Deletion; Gene Targeting; Hemostasis; Mice; Mitogen-Activated Protein Kinase 1/metabolism; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases/metabolism; Neovascularization, Physiologic; Protein Structure, Tertiary; Thromboplastin/chemistry; Thromboplastin/genetics; Thromboplastin/physiology; Thrombosis/etiology |
| public |
| Cancel |
