GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

TableEdit

Jump to: navigation, search

PMID:25348153

You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki

See Help for Help on this wiki. See the documentation for how to use the table editor

Citation

Wang, X, Sumida, H and Cyster, JG (2014) GPR18 is required for a normal CD8αα intestinal intraepithelial lymphocyte compartment. J. Exp. Med. 211:2351-9

Abstract

Intraepithelial lymphocytes (IELs) play an important role in maintaining the physiology of the small intestine. The majority of mouse IELs express CD8αα and are either γδ or αβ T cells. Although the development and homing of CD8αα IELs have been studied in some detail, the factors controlling their homeostasis and positioning are incompletely understood. Here we demonstrate that G protein-coupled receptor 18 (GPR18) is abundantly expressed in CD8αα IELs and that mice lacking this orphan receptor have reduced numbers of γδT IELs. Mixed bone marrow chimera experiments reveal a markedly reduced contribution of GPR18-deficient cells to the CD8αα IEL compartment and a reduction in the CD8αβ T cell subset. These defects could be rescued by transduction with a GPR18-expressing retrovirus. The GPR18-deficient γδT IELs that remained in mixed chimeras had elevated Thy1, and there were less granzyme B(+) and Vγ7(+) cells, indicating a greater reduction in effector-type cells. Flow cytometric analysis indicated GPR18 deficiency more strongly affected the CD8αα cells in the intraepithelial compared with the adjacent lamina propria compartment. These findings establish a requirement for GPR18 in CD8αα and CD8αβ IELs, and we suggest the receptor has a role in augmenting the accumulation of CD8 T cells in the intraepithelial versus lamina propria compartment.

Links

PubMed PMC4235638 Online version:10.1084/jem.20140646

Keywords

Animals; CD8 Antigens/immunology; CD8 Antigens/metabolism; CD8-Positive T-Lymphocytes/immunology; CD8-Positive T-Lymphocytes/metabolism; Epithelium/immunology; Epithelium/metabolism; Flow Cytometry; Gene Expression/immunology; Granzymes/immunology; Granzymes/metabolism; Intestine, Small/immunology; Intestine, Small/metabolism; Lymphocytes/immunology; Lymphocytes/metabolism; Mice, Inbred C57BL; Mice, Knockout; Microscopy, Fluorescence, Multiphoton; Receptors, Antigen, T-Cell, alpha-beta/immunology; Receptors, Antigen, T-Cell, alpha-beta/metabolism; Receptors, Antigen, T-Cell, gamma-delta/immunology; Receptors, Antigen, T-Cell, gamma-delta/metabolism; Receptors, G-Protein-Coupled/genetics; Receptors, G-Protein-Coupled/immunology; Receptors, G-Protein-Coupled/metabolism; Reverse Transcriptase Polymerase Chain Reaction; T-Lymphocyte Subsets/immunology; T-Lymphocyte Subsets/metabolism; Thy-1 Antigens/immunology; Thy-1 Antigens/metabolism

public



Cancel