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PMID:11003645

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Citation

Kipp, M, Göhring, F, Ostendorp, T, van Drunen, CM, van Driel, R, Przybylski, M and Fackelmayer, FO (2000) SAF-Box, a conserved protein domain that specifically recognizes scaffold attachment region DNA. Mol. Cell. Biol. 20:7480-9

Abstract

SARs (scaffold attachment regions) are candidate DNA elements for partitioning eukaryotic genomes into independent chromatin loops by attaching DNA to proteins of a nuclear scaffold or matrix. The interaction of SARs with the nuclear scaffold is evolutionarily conserved and appears to be due to specific DNA binding proteins that recognize SARs by a mechanism not yet understood. We describe a novel, evolutionarily conserved protein domain that specifically binds to SARs but is not related to SAR binding motifs of other proteins. This domain was first identified in human scaffold attachment factor A (SAF-A) and was thus designated SAF-Box. The SAF-Box is present in many different proteins ranging from yeast to human in origin and appears to be structurally related to a homeodomain. We show here that SAF-Boxes from four different origins, as well as a synthetic SAF-Box peptide, bind to natural and artificial SARs with high specificity. Specific SAR binding of the novel domain is achieved by an unusual mass binding mode, is sensitive to distamycin but not to chromomycin, and displays a clear preference for long DNA fragments. This is the first characterization of a specific SAR binding domain that is conserved throughout evolution and has DNA binding properties that closely resemble that of the unfractionated nuclear scaffold.

Links

PubMed PMC86301

Keywords

Amino Acid Motifs; Amino Acid Sequence; Animals; Binding Sites; Cell Line; Chromatin/genetics; Chromatin/metabolism; Chromomycins/pharmacology; Cloning, Molecular; Conserved Sequence; DNA/genetics; DNA/metabolism; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Distamycins/pharmacology; Humans; Molecular Sequence Data; Mutation; Nuclear Proteins/chemistry; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Oligodeoxyribonucleotides/genetics; Oligodeoxyribonucleotides/metabolism; Peptide Fragments/chemical synthesis; Peptide Fragments/chemistry; Peptide Fragments/metabolism; Protein Binding; Protein Structure, Tertiary; Recombinant Proteins; Sequence Homology, Amino Acid; Substrate Specificity; Transfection

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