GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
TableEdit
PMID:27718307
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
See Help for Help on this wiki. See the documentation for how to use the table editor
Citation |
Sariki, SK, Sahu, PK, Golla, U, Singh, V, Azad, GK and Tomar, RS (2016) Sen1, the homolog of human Senataxin, is critical for cell survival through regulation of redox homeostasis, mitochondrial function, and the TOR pathway in Saccharomyces cerevisiae. FEBS J. 283:4056-4083 |
---|---|
Abstract |
Mutations in the Senataxin gene, SETX are known to cause the neurodegenerative disorders, ataxia with oculomotor apraxia type 2 (AOA2), and amyotrophic lateral sclerosis 4 (ALS4). However, the mechanism underlying disease pathogenesis is still unclear. The Senataxin N-terminal protein-interaction and C-terminal RNA/DNA helicase domains are conserved in the Saccharomyces cerevisiae homolog, Sen1p. Using genome-wide expression analysis, we first show alterations in key cellular pathways such as: redox, unfolded protein response, and TOR in the yeast sen1 ΔN mutant (N-terminal truncation). This mutant exhibited growth defects on nonfermentable carbon sources, was sensitive to oxidative stress, and showed severe loss of mitochondrial DNA. The growth defect could be partially rescued upon supplementation with reducing agents and antioxidants. Furthermore, the mutant showed higher levels of reactive oxygen species, lower UPR activity, and alterations in mitochondrial membrane potential, increase in vacuole acidity, free calcium ions in the cytosol, and resistance to rapamycin treatment. Notably, the sen1 ∆N mutant showed increased cell death and shortened chronological life span. Given the strong similarity of the yeast and human Sen1 proteins, our study thus provides a mechanism for the progressive neurological disorders associated with mutations in human senataxin. |
Links |
PubMed Online version:10.1111/febs.13917 |
Keywords |
Autophagy/genetics; Cardiolipins/biosynthesis; Cellular Senescence/genetics; DNA Helicases/genetics; DNA Helicases/metabolism; Gene Expression Profiling/methods; Gene Expression Regulation, Fungal; Gene Regulatory Networks; Homeostasis/genetics; Humans; Immunoblotting; Membrane Potential, Mitochondrial/genetics; Microbial Viability/genetics; Microscopy, Fluorescence; Mitochondria/genetics; Mitochondria/metabolism; Models, Genetic; Mutation; Oxidation-Reduction; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; RNA Helicases/genetics; RNA Helicases/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Signal Transduction/genetics; Unfolded Protein Response/genetics |
public |
Cancel |