GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
TableEdit
PMID:25870259
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
See Help for Help on this wiki. See the documentation for how to use the table editor
Citation |
Rajaure, M, Berry, J, Kongari, R, Cahill, J and Young, R (2015) Membrane fusion during phage lysis. Proc. Natl. Acad. Sci. U.S.A. 112:5497-502 |
---|---|
Abstract |
In general, phages cause lysis of the bacterial host to effect release of the progeny virions. Until recently, it was thought that degradation of the peptidoglycan (PG) was necessary and sufficient for osmotic bursting of the cell. Recently, we have shown that in Gram-negative hosts, phage lysis also requires the disruption of the outer membrane (OM). This is accomplished by spanins, which are phage-encoded proteins that connect the cytoplasmic membrane (inner membrane, IM) and the OM. The mechanism by which the spanins destroy the OM is unknown. Here we show that the spanins of the paradigm coliphage lambda mediate efficient membrane fusion. This supports the notion that the last step of lysis is the fusion of the IM and OM. Moreover, data are provided indicating that spanin-mediated fusion is regulated by the meshwork of the PG, thus coupling fusion to murein degradation by the phage endolysin. Because endolysin function requires the formation of μm-scale holes by the phage holin, the lysis pathway is seen to require dramatic dynamics on the part of the OM and IM, as well as destruction of the PG. |
Links |
PubMed PMC4418876 Online version:10.1073/pnas.1420588112 |
Keywords |
Bacteriophage lambda/physiology; Cell Membrane/genetics; Cell Membrane/metabolism; Endopeptidases/genetics; Endopeptidases/physiology; Escherichia coli/genetics; Escherichia coli/virology; Membrane Fusion; Peptidoglycan/genetics; Peptidoglycan/metabolism; Viral Proteins/genetics; Viral Proteins/metabolism; Virus Release/physiology |
public |
Cancel |