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TableEdit
CAEEL:RFS1
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Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status | |
---|---|---|---|---|---|---|---|---|---|---|---|
public |
GO:0000730 |
DNA recombinase assembly |
PMID:27867009 |
ECO:0000316 |
UniProtKB:Q21621
|
P |
Figure 1 depicts the cessation of the dissociation of RAD-51 from ssDNA due to the presence of protein RFS-1/RIP-1. Figure 1, Part H [(i), (ii)] shows the known mechanism of RAD-51 by demonstrating that it’s presence prohibits the binding of ScRPA-eGFP-ssDNA. The incapability of RAD-51 to be expressed due to the lack of ATP is found in Figure 1, Part H (ii). Figure 1 ultimately illustrates that in the precence of the protein RFS-1/RIP-1 and ATP; RAD-51 along with the protein RFS-1/RIP-1 is present but the ScRPA-eGFP-ssDNA are not [(Figure 1, Part H (iii)]. The same events occur when the protein RFS-1/RIP-1 is present but not ATP [(Figure 1, Part H (iv)]. |
complete | |||
public |
GO:0000730 |
DNA recombinase assembly |
PMID:26186187 |
ECO:0000316 |
UniProtKB:Q21621
|
P |
Figure 2 provides the biochemical properties of the RFS-1/RIP-1 complex. The western blot in part A of this figure confirms the precence of the two proteins (RFS-1/RIP-1) after co-purifying the recombinant proteins from budding yeast cells. The Electrophoretic mobility shift assays (EMSA) shown in part B of the figure reveals that the RFS-1/RIP-1 complex binds in a nucleotide independent manner weakly to ssDNA but not dsDNA. Bands are observed only for ssDNA. The RFS-1/RIP-1 complex can also stimulate D loop formations with RAD51 (C) and the fact that ATP is required to do so (D). |
complete | |||
protected |
part_of |
GO:0033061 |
DNA recombinase mediator complex |
PMID:26186187 |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
protected |
enables |
GO:0051117 |
ATPase binding |
PMID:20122407 |
ECO:0000353 |
physical interaction evidence used in manual assertion |
WB:WBGene00004297 |
F |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0000712 |
resolution of meiotic recombination intermediates |
PMID:20122407 |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
WB:WBGene00021905 |
P |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0000712 |
resolution of meiotic recombination intermediates |
PMID:20122407 |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
WB:WBGene00009124 |
P |
Seeded From UniProt |
complete | |
protected |
enables |
GO:0003677 |
DNA binding |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR020588 |
F |
Seeded From UniProt |
complete | |
protected |
enables |
GO:0005524 |
ATP binding |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR020588 |
F |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0006281 |
DNA repair |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR020588 |
P |
Seeded From UniProt |
complete | |
protected |
enables |
GO:0008094 |
DNA-dependent ATPase activity |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR020588 |
F |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0006310 |
DNA recombination |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0233 |
P |
Seeded From UniProt |
complete | |
protected |
part_of |
GO:0005634 |
nucleus |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0539 |
C |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0051321 |
meiotic cell cycle |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0469 |
P |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0006281 |
DNA repair |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0234 |
P |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0006974 |
cellular response to DNA damage stimulus |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0227 |
P |
Seeded From UniProt |
complete | |
protected |
enables |
GO:0003677 |
DNA binding |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0238 |
F |
Seeded From UniProt |
complete |
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