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CAEEL:RFS1

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Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

public

GO:0000730

DNA recombinase assembly

PMID:27867009

ECO:0000316

UniProtKB:Q21621


P

Figure 1 depicts the cessation of the dissociation of RAD-51 from ssDNA due to the presence of protein RFS-1/RIP-1. Figure 1, Part H [(i), (ii)] shows the known mechanism of RAD-51 by demonstrating that it’s presence prohibits the binding of ScRPA-eGFP-ssDNA. The incapability of RAD-51 to be expressed due to the lack of ATP is found in Figure 1, Part H (ii). Figure 1 ultimately illustrates that in the precence of the protein RFS-1/RIP-1 and ATP; RAD-51 along with the protein RFS-1/RIP-1 is present but the ScRPA-eGFP-ssDNA are not [(Figure 1, Part H (iii)]. The same events occur when the protein RFS-1/RIP-1 is present but not ATP [(Figure 1, Part H (iv)].

complete
CACAO 13210

public

GO:0000730

DNA recombinase assembly

PMID:26186187

ECO:0000316

UniProtKB:Q21621


P

Figure 2 provides the biochemical properties of the RFS-1/RIP-1 complex. The western blot in part A of this figure confirms the precence of the two proteins (RFS-1/RIP-1) after co-purifying the recombinant proteins from budding yeast cells. The Electrophoretic mobility shift assays (EMSA) shown in part B of the figure reveals that the RFS-1/RIP-1 complex binds in a nucleotide independent manner weakly to ssDNA but not dsDNA. Bands are observed only for ssDNA. The RFS-1/RIP-1 complex can also stimulate D loop formations with RAD51 (C) and the fact that ATP is required to do so (D).

complete
CACAO 13251

protected

part_of

GO:0033061

DNA recombinase mediator complex

PMID:26186187

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

protected

enables

GO:0051117

ATPase binding

PMID:20122407

ECO:0000353

physical interaction evidence used in manual assertion

WB:WBGene00004297

F

Seeded From UniProt

complete

protected

involved_in

GO:0000712

resolution of meiotic recombination intermediates

PMID:20122407

ECO:0000316

genetic interaction evidence used in manual assertion

WB:WBGene00021905

P

Seeded From UniProt

complete

protected

involved_in

GO:0000712

resolution of meiotic recombination intermediates

PMID:20122407

ECO:0000316

genetic interaction evidence used in manual assertion

WB:WBGene00009124

P

Seeded From UniProt

complete

protected

enables

GO:0003677

DNA binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR020588

F

Seeded From UniProt

complete

protected

enables

GO:0005524

ATP binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR020588

F

Seeded From UniProt

complete

protected

involved_in

GO:0006281

DNA repair

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR020588

P

Seeded From UniProt

complete

protected

enables

GO:0008094

DNA-dependent ATPase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR020588

F

Seeded From UniProt

complete

protected

involved_in

GO:0006310

DNA recombination

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0233

P

Seeded From UniProt

complete

protected

part_of

GO:0005634

nucleus

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0539
UniProtKB-SubCell:SL-0191

C

Seeded From UniProt

complete

protected

involved_in

GO:0051321

meiotic cell cycle

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0469

P

Seeded From UniProt

complete

protected

involved_in

GO:0006281

DNA repair

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0234

P

Seeded From UniProt

complete

protected

involved_in

GO:0006974

cellular response to DNA damage stimulus

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0227

P

Seeded From UniProt

complete

protected

enables

GO:0003677

DNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0238

F

Seeded From UniProt

complete




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