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TableEdit
ECOLI:CRL
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Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status | |
---|---|---|---|---|---|---|---|---|---|---|---|
protected |
involved_in |
GO:0045893 |
positive regulation of transcription, DNA-templated |
PMID:17332743 |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
protected |
involved_in |
GO:0034622 |
cellular protein-containing complex assembly |
PMID:24043782 |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
protected |
part_of |
GO:0005829 |
cytosol |
PMID:18304323 |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
public |
GO:0016987 |
sigma factor activity |
PMID:9878355 |
ECO:0000314 |
F |
Transcriptional assays, as showed in Figure 5, were performed on super-coiled DNA template with and without CRP in the presence of RNA polymerase reconstituted either with wild type of different mutant of sigma factor 70. The analysis showed that CRP AR3+ activates RNA polymerase containing wild-type sigma factor 70 sixfold. However, RNA polymerase with mutant sigma factor were defective in activation by CRP AR3+. |
complete | ||||
protected |
part_of |
GO:0005829 |
cytosol |
PMID:1357528 |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
public |
GO:0016987 |
sigma factor activity |
PMID:9878355 |
ECO:0000314 |
F |
As figure 2 shows, a series of plasmids were constructed each encoding a different alanine substitution. The complementation by sigma factor 70 C-terminal alanine substitution mutants was similar to the wild-type construct, indicating that the side-chains of most residues in C terminal region are not required for the general functions of sigma factor. |
complete | ||||
protected |
involved_in |
GO:0045893 |
positive regulation of transcription, DNA-templated |
GO_REF:0000002 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR009986 |
P |
Seeded From UniProt |
complete | |
protected |
part_of |
GO:0005737 |
cytoplasm |
GO_REF:0000104 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000100858 |
C |
Seeded From UniProt |
complete | |
protected |
involved_in |
GO:0045893 |
positive regulation of transcription, DNA-templated |
GO_REF:0000104 |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000100858 |
P |
Seeded From UniProt |
complete | |
protected |
part_of |
GO:0005737 |
cytoplasm |
GO_REF:0000037 |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-0963 |
C |
Seeded From UniProt |
complete |
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