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ECOLI:CRL

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Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

protected

involved_in

GO:0045893

positive regulation of transcription, DNA-templated

PMID:17332743

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

protected

involved_in

GO:0034622

cellular protein-containing complex assembly

PMID:24043782

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

protected

part_of

GO:0005829

cytosol

PMID:18304323

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

public

GO:0016987

sigma factor activity

PMID:9878355

ECO:0000314

F

Transcriptional assays, as showed in Figure 5, were performed on super-coiled DNA template with and without CRP in the presence of RNA polymerase reconstituted either with wild type of different mutant of sigma factor 70. The analysis showed that CRP AR3+ activates RNA polymerase containing wild-type sigma factor 70 sixfold. However, RNA polymerase with mutant sigma factor were defective in activation by CRP AR3+.

complete
CACAO 11569

protected

part_of

GO:0005829

cytosol

PMID:1357528

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

public

GO:0016987

sigma factor activity

PMID:9878355

ECO:0000314

F

As figure 2 shows, a series of plasmids were constructed each encoding a different alanine substitution. The complementation by sigma factor 70 C-terminal alanine substitution mutants was similar to the wild-type construct, indicating that the side-chains of most residues in C terminal region are not required for the general functions of sigma factor.

complete
CACAO 11650

protected

involved_in

GO:0045893

positive regulation of transcription, DNA-templated

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR009986
InterPro:IPR038208

P

Seeded From UniProt

complete

protected

part_of

GO:0005737

cytoplasm

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000100858

C

Seeded From UniProt

complete

protected

involved_in

GO:0045893

positive regulation of transcription, DNA-templated

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000100858

P

Seeded From UniProt

complete

protected

part_of

GO:0005737

cytoplasm

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0963
UniProtKB-SubCell:SL-0086

C

Seeded From UniProt

complete




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