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YEAST:NOP53
Contents
| Species (Taxon ID) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). (559292) | |
| Gene Name(s) | NOP53 | |
| Protein Name(s) | Ribosome biogenesis protein NOP53
Nucleolar protein 53 | |
| External Links | ||
| UniProt | Q12080 | |
| EMBL | U43703 X96770 Z73502 BK006949 | |
| PIR | S65157 | |
| RefSeq | NP_015179.1 | |
| ProteinModelPortal | Q12080 | |
| BioGrid | 36037 | |
| DIP | DIP-993N | |
| IntAct | Q12080 | |
| MINT | MINT-8285482 | |
| iPTMnet | Q12080 | |
| MaxQB | Q12080 | |
| EnsemblFungi | YPL146C | |
| GeneID | 855957 | |
| KEGG | sce:YPL146C | |
| EuPathDB | FungiDB:YPL146C | |
| SGD | S000006067 | |
| HOGENOM | HOG000182423 | |
| InParanoid | Q12080 | |
| KO | K14840 | |
| OMA | EYEKHAW | |
| OrthoDB | EOG7J1899 | |
| BioCyc | YEAST:G3O-34043-MONOMER | |
| PRO | PR:Q12080 | |
| Proteomes | UP000002311 | |
| GO | GO:0005730 GO:0005654 GO:0005634 GO:0019843 GO:0000463 GO:0000055 GO:0006364 | |
| InterPro | IPR011687 | |
| PANTHER | PTHR14211 | |
| Pfam | PF07767 | |
| PIRSF | PIRSF017302 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0000460 |
maturation of 5.8S rRNA |
ECO:0000315 |
P |
RNA polymerase I produces pre-rRNA that is processed into several fragments, including the 5’-ETS (external transcribed spacer) and 7S pre-rRNA. The RNA exosome is a 3’ to 5’ exonuclease involved in the processing of 7S pre-rRNA into mature 5.8S rRNA and in the degradation of the 5’-ETS. The exosome requires protein cofactors that interact with the Mtr4 helicase for substrate specificity. One of these cofactors is Nop53. The N-terminus of Nop53 is required for binding with the arch domain of Mtr4, and subsequently for presentation of the 7S pre-rRNA substrate to the RNA exosome. The northern blot in Figure 1 shows that S. cerevisiae strains with wild type Mtr4 and Nop53 accumulate no 7S-pre-rRNA. Conversely, mutants with a deletion in either the arch domain of Mtr4 or the N terminus of Nop53 show accumulation of 7S-pre-rRNA. Therefore these two proteins are involved in the maturation of a precursor 5.8S ribosomal RNA (rRNA) molecule into a mature 5.8S rRNA molecule (GO:0000460) |
complete | |||||
|
part_of |
GO:0005634 |
nucleus |
ECO:0007005 |
high throughput direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000027 |
ribosomal large subunit assembly |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0019843 |
rRNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006364 |
rRNA processing |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005730 |
nucleolus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005730 |
nucleolus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000463 |
maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000055 |
ribosomal large subunit export from nucleus |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008097 |
5S rRNA binding |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000368190 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0006364 |
rRNA processing |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000368190 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005730 |
nucleolus |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
MGI:MGI:2154441 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0000027 |
ribosomal large subunit assembly |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000368190 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0042254 |
ribosome biogenesis |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006364 |
rRNA processing |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005654 |
nucleoplasm |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005730 |
nucleolus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Thoms, M et al. (2015) The Exosome Is Recruited to RNA Substrates through Specific Adaptor Proteins. Cell 162 1029-38 PubMed GONUTS page
- ↑ Kumar, A et al. (2002) Subcellular localization of the yeast proteome. Genes Dev. 16 707-19 PubMed GONUTS page
- ↑ 3.0 3.1 Granato, DC et al. (2005) Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae. FEBS J. 272 4450-63 PubMed GONUTS page
- ↑ 4.0 4.1 Rout, MP et al. (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J. Cell Biol. 148 635-51 PubMed GONUTS page
- ↑ 5.0 5.1 5.2 Thomson, E & Tollervey, D (2005) Nop53p is required for late 60S ribosome subunit maturation and nuclear export in yeast. RNA 11 1215-24 PubMed GONUTS page
- ↑ Sydorskyy, Y et al. (2005) Nop53p is a novel nucleolar 60S ribosomal subunit biogenesis protein. Biochem. J. 388 819-26 PubMed GONUTS page
- ↑ 7.0 7.1 7.2 7.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
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